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PMID: 7877988 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A diabetes-associated T-cell autoantigen maps to a telomeric locus on mouse chromosome 6.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 5 ·1995-02-28 ·Pages 1386-90

Dallas-Pedretti A, McDuffie M, Haskins K

Abstract

Identification of diabetes-associated T-cell autoantigens is important for understanding the immunopathology of diabetes and developing improved therapeutic strategies. We have used a genetic approach to move toward identifying the autoantigen recognized by a diabetogenic islet-specific T-cell clone from a nonobese diabetic (NOD) mouse. The unique antigen recognition pattern of this clone was utilized to map the gene encoding the antigen (or its expression) by genetic linkage analysis. In vitro analysis of T-cell proliferation by this clone showed that the capacity of the islets to stimulate T cells segregates as a single codominant gene in BALB/cByJ x (BALB/cByJ x NOD/Bdc) backcross mice. This phenotype was tightly linked to two microsatellites in the telomeric region of mouse chromosome 6.

MeSH Terms
Animals Autoantigens/genetics Chromosome Mapping Diabetes Mellitus, Type 1/genetics Female Genetic Linkage Genetic Markers Male Mice Mice, Inbred BALB C Mice, Inbred NOD Mice, Inbred Strains T-Lymphocytes
Chemicals
Autoantigens Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dallas-Pedretti A
Department of Immunology, University of Colorado Health Sciences Center, Denver 80262.
McDuffie M
Haskins K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.5.1386
Published
1995-02-28
Pages
1386-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42524
Subset
IM
Grants
NIDDK NIH HHS · R01 DK44132 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.5.1386 PMC Full Text View journal details
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