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PMID: 7858235 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and expression analysis of two rice genes encoding the major intrinsic protein.

Plant molecular biology ·Vol. 26 ·No. 6 ·1994-12-00 ·Pages 2003-7

Liu Q, Umeda M, Uchimiya H

Abstract

We isolated two rice cDNAs (rMip1 and rTip1) which are homologous to the genes encoding the major intrinsic protein (Mip) (soybean nod-26 and Arabidopsis gamma-Tip), respectively. Expression of rTip1 in shoots and roots of rice seedlings was enhanced by water stress, salt stress and exogenous ABA. rMip1 was expressed only in shoots. Although mRNA level of rMip1 in shoots was induced to a small extent by exogenous ABA, it did not show any increase under water or salt stress over the course of 12 h. On the basis of the differential expression patterns and evolutional distinctions, it is suggested that the possible channel proteins encoded by rMip1 and rTip1 genes may function in different transport systems.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Aquaporins Conserved Sequence Eye Proteins/genetics Genes, Plant/genetics Membrane Glycoproteins Membrane Proteins/genetics Molecular Sequence Data Oryza/drug effects,genetics Plant Proteins/biosynthesis,genetics Salts/pharmacology Sequence Homology, Amino Acid Species Specificity Tissue Distribution Water/metabolism
Chemicals
Aquaporins Eye Proteins Membrane Glycoproteins Membrane Proteins Plant Proteins Salts aquaporin 0 nodulin tonoplast intrinsic protein, plant Water Abscisic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liu Q
Cellular Function Laboratory, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Umeda M
Uchimiya H
References (16)
16 references, click to expand
  1. A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein.
    Plant Mol Biol. 1993 Dec;23(6):1187-98 PMID: 8292783
  2. An intrinsic tonoplast protein of protein storage vacuoles in seeds is structurally related to a bacterial solute transporter (GIpF).
    Plant Cell. 1990 Jun;2(6):525-32 PMID: 2152174
  3. A yeast homologue of the bovine lens fibre MIP gene family complements the growth defect of a Saccharomyces cerevisiae mutant on fermentable sugars but not its defect in glucose-induced RAS-mediated cAMP signalling.
    EMBO J. 1991 Aug;10(8):2095-104 PMID: 1648479
  4. DIP: a member of the MIP family of membrane proteins that is expressed in mature seeds and dark-grown seedlings of Antirrhinum majus.
    Plant J. 1993 Oct;4(4):717-25 PMID: 7504555
  5. The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning.
    Cell. 1984 Nov;39(1):49-59 PMID: 6207938
  6. Nodulin-26, a peribacteroid membrane nodulin is expressed independently of the development of the peribacteroid compartment.
    Nucleic Acids Res. 1987 Jan 26;15(2):813-24 PMID: 3822816
  7. Root-specific genes from tobacco and Arabidopsis homologous to an evolutionarily conserved gene family of membrane channel proteins.
    Nucleic Acids Res. 1990 Dec 25;18(24):7449 PMID: 2129561
  8. Topology and phosphorylation of soybean nodulin-26, an intrinsic protein of the peribacteroid membrane.
    J Cell Biol. 1992 Jul;118(2):481-90 PMID: 1629242
  9. Soybean nodulin-26 gene encoding a channel protein is expressed only in the infected cells of nodules and is regulated differently in roots of homologous and heterologous plants.
    Plant Cell. 1993 Jul;5(7):781-94 PMID: 7689881
  10. Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes.
    Plant Mol Biol. 1990 Jul;15(1):11-26 PMID: 1715781
  11. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11110-4 PMID: 1722319
  12. Similarity of the product of the Drosophila neurogenic gene big brain to transmembrane channel proteins.
    Nature. 1990 May 10;345(6271):163-7 PMID: 1692392
  13. Soybean nodulin 26 is homologous to the major intrinsic protein of the bovine lens fiber membrane.
    Nucleic Acids Res. 1988 Oct 11;16(19):9347 PMID: 3174457
  14. Expressed sequence tags from cultured cells of rice (Oryza sativa L.) under stressed conditions: analysis of transcripts of genes engaged in ATP-generating pathways.
    Plant Mol Biol. 1994 Jun;25(3):469-78 PMID: 8049371
  15. Vegetative and Seed-Specific Forms of Tonoplast Intrinsic Protein in the Vacuolar Membrane of Arabidopsis thaliana.
    Plant Physiol. 1992 Jun;99(2):561-70 PMID: 16668923
  16. The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes.
    EMBO J. 1993 Jun;12(6):2241-7 PMID: 8508761
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019511
Published
1994-12-00
Pages
2003-7
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
D17443, D25534
External Links
PubMed source DOI: 10.1007/BF00019511 View journal details
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