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PMID: 7854312 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast.

Molecular & general genetics : MGG ·Vol. 246 ·No. 3 ·1995-02-06 ·Pages 269-81

Takita Y, Ohya Y, Anraku Y

Abstract

Genetic screening of Saccharomyces cerevisiae mutants defective in Ca2+ homeostasis identified cls2, which exhibits a specific Ca(2+)-sensitive growth phenotype. We describe here the CLS2 gene and a multicopy suppressor (named BCL21, for bypass of CLS2) of the cls2 mutation. The CLS2 gene encodes a polypeptide of 410 amino acid residues, and its hydropathy profile indicates that the predicted Cls2 protein (Cls2p) contains ten putative membrane spanning regions. Immunofluorescent staining of the yeast cells expressing epitope-tagged Cls2p suggests that Cls2p is localized to endoplasmatic reticulum (ER) membrane. A cls2 disruption strain is viable, but shows a Ca(2+)-sensitive phenotype like the original cls2 mutants. BCL21 suppresses the cls2 disruption mutation, indicating that the multicopy suppression does not require the Cls2p. Suppression of cls2 was observed even after introduction of a single-copy plasmid harboring BCL21. The BCL21 gene encodes a protein of 382 amino acid residues and is identical to the SUR1 gene. sur1 was originally isolated as a suppressor of rvs161, which has reduced viability in nutrient starvation conditions. Possible mechanisms of the multicopy suppression are discussed.

Related Genes
BCL21 CLS2
MeSH Terms
Amino Acid Sequence Base Sequence Calcium/metabolism Calcium-Binding Proteins/analysis,genetics,physiology Cloning, Molecular Endoplasmic Reticulum/chemistry Fungal Proteins/genetics,physiology Genes, Fungal/genetics Genes, Suppressor/genetics Genetic Complementation Test Glycosyltransferases Membrane Proteins/genetics,physiology Models, Biological Molecular Sequence Data Mutation/physiology Repressor Proteins/genetics Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
CSG2 protein, S cerevisiae Calcium-Binding Proteins Fungal Proteins Membrane Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Glycosyltransferases SUR1 protein, S cerevisiae Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Takita Y
Department of Plant Sciences, Graduate School of Science, University of Tokyo, Japan.
Ohya Y
Anraku Y
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00288599
Published
1995-02-06
Pages
269-81
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
D26581, D28120
External Links
PubMed source DOI: 10.1007/BF00288599
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