Home LiteratureArticle Details
PMID: 785221 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Insertion sequence IS2 near the gene for prophage lambda excision.

Molecular & general genetics : MGG ·Vol. 147 ·No. 1 ·1976-08-10 ·Pages 103-9

Mosharrafa E, Pilacinski W, Zissler J, Fiandt M, Szybalski W

Abstract

In this study we characterize a variant of the lambdacI857S7 prophage, designated lambdabi2cI857S7, which carries a DNA insertion. The insertion sequence is IS2, and it resides in the antipolar orientation II just upstream from the gene for prophage excision (xis) at 61.6%lambda. This bi2 insertion mutant could prove valuable for studies on possible recombination functions of IS2 DNA and of its effect on the lambda integration and excision functions.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Coliphages DNA, Viral Electrophoresis, Agar Gel Escherichia coli Microscopy, Electron Nucleic Acid Conformation
Chemicals
DNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mosharrafa E
Pilacinski W
Zissler J
Fiandt M
Szybalski W
References (36)
36 references, click to expand
  1. Physical mapping of coliphage lambda att2.
    Virology. 1976 Jul 1;72(1):6-12 PMID: 779246
  2. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
    Virology. 1969 May;38(1):200-2 PMID: 4891223
  3. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
    Science. 1969 Mar 21;163(3873):1343-8 PMID: 5765116
  4. Polar mutations in lac, gal and phage lambda consist of a few IS-DNA sequences inserted with either orientation.
    Mol Gen Genet. 1972;119(3):223-31 PMID: 4567156
  5. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
    J Virol. 1974 Nov;14(5):1235-44 PMID: 4372397
  6. O0 and strong-polar mutations in the gal operon are insertions.
    Mol Gen Genet. 1968;102(4):353-63 PMID: 5743437
  7. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534-8 PMID: 4601822
  8. Mutations of coliphage lambda affecting the expression of replicative functions O and P.
    Mol Gen Genet. 1970;108(3):266-76 PMID: 5475574
  9. Electron microscopy of polar insertions in the lac operon of Escherichia coli.
    Mol Gen Genet. 1972;119(3):207-22 PMID: 4567155
  10. Recombination-deficient deletions in bacteriophage lambda and their interaction with chi mutations.
    Genetics. 1975 Feb;79(2):143-74 PMID: 1093930
  11. Crypticogenicity of bacteriophage lambda.
    J Mol Biol. 1970 Jun 14;50(2):481-90 PMID: 4920426
  12. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.
    J Mol Biol. 1975 Oct 5;97(4):561-75 PMID: 1102715
  13. On the process of cellular division in Escherichia coli: replication of the bacterial chromosome under control of prophage P2.
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2407-11 PMID: 4944622
  14. Frameshift mutations in the lactose operon of E. coli.
    Cold Spring Harb Symp Quant Biol. 1966;31:189-201 PMID: 4866375
  15. The cryptogenic factor in lambda.
    Virology. 1968 Apr;34(4):650-63 PMID: 5650699
  16. Mutations caused by the insertion of genetic material into the galactose operon of Escherichia coli.
    J Mol Biol. 1969 Feb 28;40(1):93-105 PMID: 4903362
  17. Insertion mutations in the control region of the Gal operon of E. coli. I. Biological characterization of the mutations.
    Mol Gen Genet. 1972;115(3):258-65 PMID: 4555679
  18. Transposition of R factor genes to bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32 PMID: 1059152
  19. Strong-polar mutations in the transferase gene of the galactose operon in E.coli.
    Mol Gen Genet. 1967;100(3):296-306 PMID: 4869076
  20. Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance.
    Virology. 1974 Dec;62(2):458-71 PMID: 4432374
  21. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.
    Mol Gen Genet. 1973 May 9;122(3):267-77 PMID: 4577900
  22. Physical mapping of bacteriophage P2 mutations and their relation to the genetic map.
    Mol Gen Genet. 1975;136(2):139-49 PMID: 16094980
  23. A TYPE OF LAMBDA PROPHAGE UNABLE TO CONFER IMMUNITY.
    Virology. 1964 Jun;23:209-16 PMID: 14187909
  24. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  25. Two kinds of insertions in bacterial genes.
    Mol Gen Genet. 1972;119(3):191-206 PMID: 4567154
  26. Electron microscope heteroduplex studies of sequence relations among bacterial plasmids: identification and mapping of the insertion sequences IS1 and IS2 in F and R plasmids.
    J Bacteriol. 1975 May;122(2):764-75 PMID: 1092668
  27. Physical mapping of the att-N region of coliphage lambda: apparent oversaturation of coding capacity in the gam-ral segment.
    Biochimie. 1974;56(11-12):1497-503 PMID: 4466498
  28. Occurrence of insertion sequence (IS) regions on plasmid deoxyribonucleic acid as direct and inverted nucleotide sequence duplications.
    J Bacteriol. 1975 May;122(2):776-81 PMID: 1092669
  29. Functional behavior of lambda cry.
    J Mol Biol. 1968 Mar 28;32(3):638-58 PMID: 4868762
  30. The galactose operon of E. coli K-12. I. Structural and pleiotropic mutations of the operon.
    Genetics. 1969 Jun;62(2):231-47 PMID: 4905871
  31. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi.
    J Mol Biol. 1971 Mar 14;56(2):403-23 PMID: 5551396
  32. IS1 and IS2 mutations in the ribosomal protein genes of E. coli K-12.
    Mol Gen Genet. 1975 Nov 3;141(1):85-9 PMID: 765727
  33. IS2, a genetic element for turn-off and turn-on of gene activity in E. coli.
    Mol Gen Genet. 1974;132(4):265-89 PMID: 4610339
  34. alphabeta sequence of F is IS31.
    J Bacteriol. 1975 Aug;123(2):687-92 PMID: 1097422
  35. Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes in E. coli.
    Nature. 1975 Jul 17;256(5514):183-7 PMID: 1097938
  36. Int-constitutive mutants of bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):237-41 PMID: 4521054
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337943
Published
1976-08-10
Pages
103-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337943
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2