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PMID: 7844141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

PICK1: a perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system.

The Journal of cell biology ·Vol. 128 ·No. 3 ·1995-02-00 ·Pages 263-71

Staudinger J, Zhou J, Burgess R, Elledge SJ, Olson EN

Abstract

Protein kinase C (PKC) plays a central role in the control of proliferation and differentiation of a wide range of cell types by mediating the signal transduction response to hormones and growth factors. Upon activation by diacylglycerol, PKC translocates to different subcellular sites where it phosphorylates numerous proteins, most of which are unidentified. We used the yeast two-hybrid system to identify proteins that interact with activated PKC alpha. Using the catalytic region of PKC fused to the DNA binding domain of yeast GAL4 as "bait" to screen a mouse T cell cDNA library in which cDNA was fused to the GAL4 activation domain, we cloned several novel proteins that interact with C-kinase (PICKs). One of these proteins, designated PICK1, interacts specifically with the catalytic domain of PKC and is an efficient substrate for phosphorylation by PKC in vitro and in vivo. PICK1 is localized to the perinuclear region and is phosphorylated in response to PKC activation. PICK1 and other PICKs may play important roles in mediating the actions of PKC.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins/genetics,isolation & purification,metabolism Cell Cycle Proteins Cell Line Cell Nucleus/metabolism DNA, Complementary Mice Molecular Sequence Data Nuclear Proteins/genetics,isolation & purification,metabolism Phosphorylation Protein Kinase C/metabolism RNA, Messenger/genetics,metabolism Saccharomyces cerevisiae/genetics Substrate Specificity
Chemicals
Carrier Proteins Cell Cycle Proteins DNA, Complementary Nuclear Proteins Prkcabp protein, mouse RNA, Messenger Protein Kinase C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Staudinger J
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Zhou J
Burgess R
Elledge S J
Olson E N
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.128.3.263
Published
1995-02-00
Pages
263-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120344
Subset
IM
Databases
GENBANK
Z46720
External Links
PubMed source DOI: 10.1083/jcb.128.3.263 PMC Full Text View journal details
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