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PMID: 7822042 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Karyotype analysis of virulent Plasmodium falciparum strains propagated in Saimiri sciureus: strain adaptation leads to deletion of the RESA gene.

Infection and immunity ·Vol. 63 ·No. 2 ·1995-02-00 ·Pages 693-5

Hinterberg K, Muanza K, Hernandez-Rivas R, Gay F, Gysin J, Mattei D, Scherf A

Abstract

The squirrel monkey, Saimiri sciureus, is an important experimental model for the study of the human malaria parasite Plasmodium falciparum. A detailed karyotype analysis of four different P. falciparum strains propagated in S. sciureus was done using various subtelomeric antigen gene probes. We observed deletion of the complete RESA gene from chromosome 1 in all four strains. Interestingly, a loss of RESA was observed immediately upon adaptation to the squirrel monkey, suggesting that this DNA rearrangement is fundamental for the P. falciparum infection of S. sciureus erythrocytes. However, a RESA-specific gene probe hybridized with chromosome 1 of wild isolates from 28 different patients, indicating that this gene is maintained during infection of humans.

MeSH Terms
Animals Antigens, Protozoan/genetics Antigens, Surface/genetics Chromosome Mapping Gene Deletion Genes, Protozoan Karyotyping Malaria/parasitology Plasmodium falciparum/genetics,pathogenicity Protozoan Proteins/genetics Saimiri
Chemicals
Antigens, Protozoan Antigens, Surface Protozoan Proteins ring-infected erythrocyte surface antigen (RESA), Plasmodium falciparum
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hinterberg K
Unité de Parasitologie Expérimentale, Centre National de la Recherche Scientifique URA 361, Institut Pasteur, Paris.
Muanza K
Hernandez-Rivas R
Gay F
Gysin J
Mattei D
Scherf A
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.2.693-695.1995
Published
1995-02-00
Pages
693-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173052
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.63.2.693-695.1995 PMC Full Text View journal details
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