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PMID: 7816559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Volume-activated Cl- currents in different mammalian non-excitable cell types.

Pflugers Archiv : European journal of physiology ·Vol. 428 ·No. 3-4 ·1994-10-00 ·Pages 364-71

Nilius B, Sehrer J, Viana F, De Greef C, Raeymaekers L, Eggermont J, Droogmans G

Abstract

The existence and properties of volume-activated Cl- currents were studied in 15 different cell types (endothelium: human umbilical vein, human aorta, bovine pulmonary artery; fibroblasts: Swiss 3T3, L, C3H 10T1/2 and COS-1; epithelium: KB3, HeLa and A6; blood cells: RBL-2H3 and Jurkat; endothelioma cells derived from both subcutaneous and thymic hemangiomas; skin: IGR1 melanoma). Volume-activated Cl- currents with common characteristics, i.e. small conductance, outward rectification, higher permeability for iodide than for chloride and sensitivity to block by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) could be elicited in all cells. The block of this current by tamoxifen and dideoxyforskolin is different for the various cell types, as well as the time course and the amplitude of the responses induced by repetitive applications of hypotonicity. Volume-activated Cl- channels with similar biophysical properties are therefore wide-spread among mammalian cells. This may reflect either a single Cl- channel that is ubiquitously expressed or a family of functionally related Cl- channels with cell specific expression patterns.

MeSH Terms

Animals Anura Cell Line Cell Physiological Phenomena Chlorides/physiology Colforsin/analogs & derivatives,pharmacology Electric Conductivity Endothelium, Vascular/cytology,physiology Epithelial Cells Epithelium/metabolism Fibroblasts/physiology Humans Iodides/metabolism Mammals Neoplasms/physiopathology Nitrobenzoates/pharmacology Permeability Tamoxifen/pharmacology

Chemicals

Chlorides Iodides Nitrobenzoates Tamoxifen Colforsin 5-nitro-2-(3-phenylpropylamino)benzoic acid 1,9-dideoxyforskolin

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Nilius B

KU Leuven, Laboratorium voor Fysiologie, Belgium.

Sehrer J

Viana F

De Greef C

Raeymaekers L

Eggermont J

Droogmans G

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21 references, click to expand

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Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/BF00724520

Published

1994-10-00

Pages

364-71

Language

English

Region

Germany

NLM ID

0154720

Subset

External Links

PubMed source DOI: 10.1007/BF00724520 View journal details

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Volume-activated Cl- currents in different mammalian non-excitable cell types.

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