Abstract
We evaluated the stability of human immunodeficiency virus (HIV) load markers from blood samples collected in VACUTAINER CPT or standard VACUTAINER brand tubes using sodium heparin or sodium citrate as anticoagulants. Quantitative plasma culture and p24 antigen concentrations were determined, and HIV RNA levels in plasma were measured by both reverse transcription-PCR-enzyme-linked immunosorbent assay (RT-PCR-ELISA) and branched DNA methods. All tubes were stored at room temperature for analysis at 2, 24, 48, and 72 h after the blood samples were drawn. No difference was seen between tube types with respect to the HIV titer in plasma or the positivity rate for all samples that demonstrated a fall in titer over time. Unbound p24 antigen levels in plasma decreased during the initial 48-h period in both tube types. Immune complex-dissociated p24 antigen levels decreased in CPT tubes but not in standard VACUTAINER tubes. The HIV RNA copy number in plasma measured by RT-PCR-ELISA was stable in most subjects and was significantly higher in CPT tubes than in standard VACUTAINER tubes at 24 and 72 h after the blood samples were drawn. The branched DNA probe assay detected a significant decline in HIV RNA equivalent in plasma over 72 h in both collection tubes, the decline being more dramatic in the standard VACUTAINER tube than the CPT tube. Overall, interday variability suggests that samples collected for a particular assay should be processed at the same time after blood is drawn and that a particular tube type be used throughout a given study.
MeSH Terms
Anticoagulants
Blood Specimen Collection/instrumentation
CD4 Lymphocyte Count
Culture Media
DNA, Viral/blood
Didanosine/therapeutic use
Drug Therapy, Combination
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
False Negative Reactions
Gels
HIV Core Protein p24/isolation & purification
HIV Infections/blood,drug therapy,microbiology
HIV-1/growth & development,isolation & purification
Humans
Polyesters
Polymerase Chain Reaction
Predictive Value of Tests
RNA, Viral/isolation & purification
Severity of Illness Index
Specimen Handling
Viremia/microbiology
Virus Cultivation
Zidovudine/therapeutic use
Chemicals
Anticoagulants
Culture Media
DNA, Viral
Gels
HIV Core Protein p24
Polyesters
RNA, Viral
Zidovudine
Didanosine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mole L
ARC-AIDS Research Center, Veterans Affairs Medical Center, Palo Alto, California 94304.
Margolis D
Carroll R
Todd J
Holodniy M
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