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PMID: 7814478 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Use of various genetic markers in differentiation of Mycobacterium bovis strains from animals and humans and for studying epidemiology of bovine tuberculosis.

Journal of clinical microbiology ·Vol. 32 ·No. 10 ·1994-10-00 ·Pages 2425-33

van Soolingen D, de Haas PE, Haagsma J, Eger T, Hermans PW, Ritacco V, Alito A, van Embden JD

Abstract

One hundred fifty-three Mycobacterium bovis strains from cattle, various animal species from zoos and wild parks, and humans were analyzed for three different genetic markers for use in the epidemiology of bovine tuberculosis. M. bovis strains isolated from cattle were found to carry a single IS6110 element, whereas the majority of strains from other animals such as antelopes, monkeys, and seals harbored multiple IS6110 elements, suggesting that the reservoirs in cattle and wild animals are separated. Because the single IS6110 element in cattle strains is located at the same chromosomal position, strain differentiation by insertion sequence fingerprinting was hampered. Therefore, we investigated the usefulness of the direct repeat and polymorphic GC-rich repeat elements for strain differentiation. Both markers allowed sufficient strain discrimination for epidemiological purposes. Evidence is presented that in Argentina, most human M. bovis infections are due to transmission from cattle, whereas M. bovis infections among humans in the Netherlands are mainly contracted from animals other than cattle. Various outbreaks of M. bovis among animals and humans are described, including a small one which likely involved transmission from human to human.

MeSH Terms
Animals Cattle DNA Fingerprinting Genetic Markers Humans Mycobacterium bovis/classification,genetics Repetitive Sequences, Nucleic Acid Tuberculosis, Bovine/microbiology
Chemicals
Genetic Markers
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
van Soolingen D
Laboratory for Bacteriology and Antimicrobial Agents, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
de Haas P E
Haagsma J
Eger T
Hermans P W
Ritacco V
Alito A
van Embden J D
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.32.10.2425-2433.1994
Published
1994-10-00
Pages
2425-33
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC264079
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.32.10.2425-2433.1994 PMC Full Text View journal details
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