Characterization and expression of a P-450-like mycinamicin biosynthesis gene using a novel Micromonospora-Escherichia coli shuttle cosmid vector.

Molecular & general genetics : MGG ·Vol. 245 ·No. 4 ·1994-11-15 ·Pages 456-64

Inouye M, Takada Y, Muto N, Beppu T, Horinouchi S

Abstract

A 29 kb shuttle cosmid vector, pTYS507, was constructed from a cryptic Micromonospora griseorubida plasmid and the Escherichia coli cosmid pJB8. Subcloning of mycinamicin II biosynthesis genes in pTYS507 led to the identification of a DNA region that could complement a mutant of M. griseorubida that lacked both hydroxylase and epoxidase activities. Nucleotide sequence and mutational analysis suggested that a single P-450-like protein catalyzes both reactions.

Related Genes

mycG

MeSH Terms

Amino Acid Sequence Anti-Bacterial Agents/biosynthesis Bacterial Proteins Base Sequence Cloning, Molecular Cosmids Cytochrome P-450 Enzyme System/genetics,metabolism DNA Mutational Analysis DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Genetic Vectors Hydroxylation Macrolides Micromonospora/enzymology,genetics Molecular Sequence Data Oxidoreductases/metabolism Restriction Mapping

Chemicals

Anti-Bacterial Agents Bacterial Proteins DNA, Bacterial Macrolides mycinamicins Cytochrome P-450 Enzyme System Oxidoreductases epoxidase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Inouye M

Institute for Life Science Research, Shizuoka, Japan.

Takada Y

Muto N

Beppu T

Horinouchi S

References (27)

27 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00302258

Published

1994-11-15

Pages

456-64

Language

English

Region

Germany

NLM ID

0125036

Subset

Databases

GENBANK

D16098

External Links

PubMed source DOI: 10.1007/BF00302258

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Characterization and expression of a P-450-like mycinamicin biosynthesis gene using a novel Micromonospora-Escherichia coli shuttle cosmid vector.

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