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PMID: 780343 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation and characterization of conjugation-deficient mutants of Escherichia coli K-12.

Journal of bacteriology ·Vol. 126 ·No. 3 ·1976-06-00 ·Pages 1194-206

Falkinham JO, Curtiss R

Abstract

Conjugation-deficient mutants (Con-) of Escherichia coli K-12 have been isolated by a variety of indirect selective techniques. Mutants with mutations conferring ampicillin resistance, fosfomycin resistance, an alanine requirement, and a failure of ferment a number of carbohydrates were selected because the impaired functions occur in association with cell wall and cell membrane defects. The integrity of these catalytic or structural elements is postulated to have a role in conjugation. The mutants could be divided into at least six general categories corresponding to their defectiveness in the following postulated recipient cell functions: (i) specific-union formation, (ii) effective-union formation, (iii) deoxyribonucleic acid transfer, (iv) plasmid establishment, (v) plasmid maintenance, and (vi) recombination. The availability of these mutants should contribute to the description of the molecular events involved in each of these conjugation steps and the elucidation of these genetic control over the inheritance of conjugationally transferred deoxyribonucleic acid.

MeSH Terms
Alanine/metabolism Ampicillin/pharmacology Carbohydrate Metabolism Conjugation, Genetic DNA, Bacterial/metabolism Escherichia coli/drug effects,isolation & purification,metabolism Fermentation Fosfomycin/pharmacology Glutamates/metabolism Mutation Penicillin Resistance Plasmids
Chemicals
DNA, Bacterial Glutamates Fosfomycin Ampicillin Alanine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Falkinham J O
Curtiss R
References (39)
39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.3.1194-1206.1976
Published
1976-06-00
Pages
1194-206
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233144
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.3.1194-1206.1976 PMC Full Text View journal details
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