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PMID: 7798309 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Facilitated nuclear transport of calmodulin in tissue culture cells.

The Journal of cell biology ·Vol. 127 ·No. 6 Pt 1 ·1994-12-00 ·Pages 1527-36

Pruschy M, Ju Y, Spitz L, Carafoli E, Goldfarb DS

Abstract

Calmodulin (CaM) potentiates Ca(2+)-dependent signaling pathways in both the cytoplasm and nucleus. We have investigated the mechanism of CaM nuclear transport using tissue culture cell microinjection and a permeabilized cell import assay. The inhibition of CaM import by the translocation inhibitor wheat germ agglutinin (WGA) and by chilling, indicates that CaM import is facilitated, but because ATP depletion does not affect CaM import, the mechanism does not appear to be active. Chilling and WGA arrest persist in ATP-depleted cells, indicating that CaM is not retained in the cytoplasm by an ATP-dependent mechanism. In permeabilized cells, both Ca(2+)-CaM and Ca(2+)-free CaM are sensitive to extract-dependent WGA and chilling import inhibition. Titration experiments in microinjected and permeabilized cells indicate that a saturable cytosolic factor(s) mediates chilling and WGA arrest.

MeSH Terms
Adenosine Triphosphate/metabolism Biological Transport/drug effects Calbindins Calmodulin/metabolism Cell Compartmentation Cell Membrane Permeability Cell Nucleus/metabolism Cells, Cultured Cold Temperature Histocytochemistry Microinjections Microscopy, Fluorescence Peptide Fragments/metabolism S100 Calcium Binding Protein G/metabolism Troponin/metabolism Troponin C Wheat Germ Agglutinins/pharmacology
Chemicals
Calbindins Calmodulin Peptide Fragments S100 Calcium Binding Protein G Troponin Troponin C Wheat Germ Agglutinins Adenosine Triphosphate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pruschy M
Institute für Biochemie III, ETH-Zentrum, CH-8092 Zürich.
Ju Y
Spitz L
Carafoli E
Goldfarb D S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.127.6.1527
Published
1994-12-00
Pages
1527-36
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120293
Subset
IM
Grants
NIGMS NIH HHS · GM40362 · United States
External Links
PubMed source DOI: 10.1083/jcb.127.6.1527 PMC Full Text View journal details
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