Home LiteratureArticle Details

PMID: 7798145 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.

Journal of bacteriology ·Vol. 177 ·No. 1 ·1995-01-00 ·Pages 271-4

Martinussen J, Glaser P, Andersen PS, Saxild HH

Abstract

Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other organisms, whereas the other gene with a low level of homology to other UPRTases turned out to be the pyrR gene--the repressor of the pyr operon. The role of these genes in uracil metabolism was established by an analysis of the phenotypes of upp and pyrR mutants.

Related Genes

pyrR upp

MeSH Terms

Amino Acid Sequence Bacillus subtilis/enzymology,genetics Bacterial Proteins Genes, Bacterial/genetics Molecular Sequence Data Pentosyltransferases/genetics Repressor Proteins/genetics Sequence Homology, Amino Acid Uracil/metabolism

Chemicals

Bacterial Proteins Repressor Proteins Uracil Pentosyltransferases PyrR protein, bacteria uracil phosphoribosyltransferase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Martinussen J

Center for Lactic Acid Bacteria, Technical University of Denmark, Lyngby.

Glaser P

Andersen P S

Saxild H H

References (10)

10 references, click to expand

An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis.
Nucleic Acids Res. 1983 Nov 25;11(22):8019-30 PMID: 6316281
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem. 1991 May 15;266(14):9113-27 PMID: 1709162
Characterization of the upp gene encoding uracil phosphoribosyltransferase of Escherichia coli K12.
Eur J Biochem. 1992 Feb 15;204(1):51-6 PMID: 1371255
Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake.
Mol Gen Genet. 1972;118(3):235-45 PMID: 4343250
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol. 1994 Jun;176(12):3708-22 PMID: 8206849
The ftf gene encoding the cell-bound fructosyltransferase of Streptococcus salivarius ATCC 25975 is preceded by an insertion sequence and followed by FUR1 and clpP homologues.
J Gen Microbiol. 1993 May;139(5):913-20 PMID: 8393068
Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12.
Biochim Biophys Acta. 1986 Apr 11;881(2):268-75 PMID: 3513846
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs.
J Bacteriol. 1987 Jul;169(7):2977-83 PMID: 3110131
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.177.1.271-274.1995

Published

1995-01-00

Pages

271-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC176586

Subset

Databases

GENBANK

Z38002

External Links

PubMed source DOI: 10.1128/jb.177.1.271-274.1995 PMC Full Text View journal details

Analysis Services

Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.

Contact

WeChat Official Account