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PMID: 7789770 Published · ppublish English Journal Article

A genetic analysis of the 63E-64A genomic region of Drosophila melanogaster: identification of mutations in a replication factor C subunit.

Genetics ·Vol. 139 ·No. 4 ·1995-04-00 ·Pages 1701-9

Harrison SD, Solomon N, Rubin GM

Abstract

We have performed an F2 genetic screen to identify lethal mutations within the 63E-64A genomic region. We have isolated 122 mutations in 20 different complementation groups. Of these groups, 16 are represented by multiple alleles. We have also established that the Rop and Ras2 genes are located within the 63E-64A genomic domain at 64A10,11. We have sequenced 10.2 kb of DNA surrounding this gene pair and find that in addition to Rop and Ras2 there is another gene located within this DNA sequence. The gene product, which we have named Rfc40, shows 68% identity to the 40-kDa subunit of replication factor C. We find that the members of one complementation group (13 alleles) derived from our screen correspond to mutations in the Rop gene, whereas the members of another (five alleles) correspond to mutations in the Rfc40 gene. In addition we have isolated 11 new mutant alleles of the disembodied gene.

Related Genes
Ras2 Rfc40 Rop
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Replication DNA-Binding Proteins/genetics Drosophila melanogaster/genetics Genes, Insect Genes, Lethal Genetic Complementation Test Heterozygote Homeodomain Proteins Homozygote Minor Histocompatibility Antigens Molecular Sequence Data Mutation Oligodeoxyribonucleotides Proto-Oncogene Proteins c-bcl-2 Replication Protein C Repressor Proteins Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
BCL2-related protein A1 DNA-Binding Proteins Homeodomain Proteins MATA1 protein, S cerevisiae Minor Histocompatibility Antigens Oligodeoxyribonucleotides Proto-Oncogene Proteins c-bcl-2 Repressor Proteins Saccharomyces cerevisiae Proteins Replication Protein C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harrison S D
Howard Hughes Medical Institute, University of California, Berkeley 94720-3200, USA.
Solomon N
Rubin G M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/139.4.1701
Published
1995-04-00
Pages
1701-9
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206495
Subset
IM
Databases
GENBANK
U15967
External Links
PubMed source DOI: 10.1093/genetics/139.4.1701 PMC Full Text View journal details
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