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PMID: 7788721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro self-splicing reactions of chloroplast and mitochondrial group-I introns in Chlamydomonas eugametos and Chlamydomonas moewusii.

Current genetics ·Vol. 27 ·No. 2 ·1995-01-00 ·Pages 177-83

Côté MJ, Turmel M

Abstract

The self-splicing activity of nine chloroplast group-I introns (CeLSU.1 to CeLSU.6, CepsbC.1, CepsbC.2 and CmpsaB.1) and of one mitochondrial group-I intron (CmmtLSU.1) from the interfertile green algae Chlamydomonas eugametos and C. moewusii was examined using RNA templates produced by in vitro transcription of cloned DNA sequences. All introns, with the exception of the mobile intron CeLSU.5 encoding the site-specific I-CeuI endonuclease, were found to catalyze their own splicing in the absence of proteins. The introns that proved to be the best substrates under the conditions employed are CeLSU.1, CeLSU.3, CeLSU.4, CepsbC.1 and CmmtLSU.1. The implications of our results for the origin and spread of group-I introns in the organellar genomes of green algae are discussed.

MeSH Terms
Animals Base Sequence Biological Evolution Chlamydomonas/genetics DNA, Chloroplast/genetics DNA, Mitochondrial/genetics Introns Molecular Sequence Data RNA Splicing RNA, Messenger/genetics Transcription, Genetic
Chemicals
DNA, Chloroplast DNA, Mitochondrial RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Côté M J
Département de biochimie, Faculté des sciences et de génie, Université Laval, Québec, Canada.
Turmel M
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00313432
Published
1995-01-00
Pages
177-83
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00313432 View journal details
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