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PMID: 778855 Published · ppublish English Journal Article

Biogenesis of mitochondria: molecular mapping of the mitochondrial genome of yeast.

Linnane AW, Lukins HB, Molloy PL, Nagley P, Rytka J, Sriprakash KS, Trembath MK

Abstract

We have developed a new procedure for the detailed molecular mapping of any allele of the yeast (Saccharomyces cerevisiae) mitochondrial genome. The procedure employs a collection of different genetically characterized petite strains whose genomes have been physically defined by molecular hybridization. The map position of an allele is within the DNA segment common to all defined petites that can be shown by marker rescue to retain the locus. The same collection of petites can be used to locate the positions of mitochondrial rRNA and tRNA cistrons and DNA fragments produced by restriction endonucleases.

MeSH Terms
Chromosome Mapping DNA, Mitochondrial Mitochondria Nucleic Acid Hybridization Recombination, Genetic Saccharomyces cerevisiae/ultrastructure
Chemicals
DNA, Mitochondrial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Linnane A W
Lukins H B
Molloy P L
Nagley P
Rytka J
Sriprakash K S
Trembath M K
References (15)
15 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1976-06-00
Pages
2082-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430453
Subset
IM
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