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PMID: 7769140 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo activity and in vitro specificity of CD4+ Th1 and Th2 cells derived from the spleens of diabetic NOD mice.

The Journal of clinical investigation ·Vol. 95 ·No. 6 ·1995-06-00 ·Pages 2979-85

Healey D, Ozegbe P, Arden S, Chandler P, Hutton J, Cooke A

Abstract

CD4+ T cell lines were generated from the spleens of diabetic NOD mice against crude membrane preparations derived from a rat insulinoma. Adoptive transfer of these lines into neonatal mice confirms that overt diabetes is induced by gamma-IFN-secreting Th1 cells, whereas transfer of IL-4-secreting Th2 cells resulted in a nondestructive peri-islet insulitis. Analysis of the antigens recognized by individual T cell clones from the Th1 line included reactivity against an insulinoma membrane fraction enriched in proteins of approximately 38 kD. Immune responses to the same antigen preparation have been associated with T cell clones derived from human insulin-dependent diabetes mellitus. The specificity of Th2 cells includes reactivity to a fraction enriched in proteins of 30 kD. The data suggest that in insulin-dependent diabetes mellitus the balance between beta cell destruction, associated with intra-islet infiltration, and nondestructive (potential protective) peri-islet insulitis may depend on both the antigens recognized, and the prevailing cytokine environment.

MeSH Terms
Animals Autoantigens/chemistry,immunology CD4-Positive T-Lymphocytes/immunology Diabetes Mellitus, Type 1/immunology Immunity, Cellular Islets of Langerhans/immunology Mice Mice, Inbred NOD/immunology Molecular Weight Proteins/chemistry,immunology Spleen/cytology Th1 Cells/immunology Th2 Cells/immunology
Chemicals
Autoantigens Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Healey D
Department of Pathology, Cambridge University, United Kingdom.
Ozegbe P
Arden S
Chandler P
Hutton J
Cooke A
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32 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI118006
Published
1995-06-00
Pages
2979-85
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC295987
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1172/JCI118006 PMC Full Text View journal details
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