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PMID: 7765565 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

The behavior of bacteria designed for biodegradation.

Bio/technology (Nature Publishing Company) ·Vol. 12 ·No. 13 ·1994-12-00 ·Pages 1349-56

Ramos JL, Díaz E, Dowling D, de Lorenzo V, Molin S, O'Gara F, Ramos C, Timmis KN

Abstract

Mineralization of organic molecules by microbes is essential for the carbon cycle to operate. The massive mobilization of compounds stored in natural resources, or the introduction of xenobiotics into the biosphere, leads to unidirectional fluxes, which result in the persistance of a number of chemicals in the biosphere, and thus constitute a source of pollution. Molecular biology offers the tools to optimize the biodegradative capacities of microorganisms, accelerate the evolution of "new" activities, and construct totally "new" pathways through the assemblage of catabolic segments from different microbes. Although the number of genetically engineered microbes (GEMs) for potential use in biodegradation is not large, these recombinant microbes function in microcosms according to their design. The survival and fate of recombinant microbes in different ecological niches under laboratory conditions is similar to what has been observed for the unmodified parental strains. rDNA, both on plasmids and on the host chromosome, is usually stably inherited by GEMs. The potential lateral transfer of rDNA from the GEMs to other microbes is significantly diminished, though not totally inhibited, when rDNA is incorporated on the host chromosome. The behavior and fate of GEMs can be predicted more accurately through the coupling of regulatory circuits that control the expression of catabolic pathways to killing genes, so that the GEMs survive in polluted environments, but die when the target chemical is eliminated.

MeSH Terms
Bacteria/genetics,metabolism Biodegradation, Environmental Gene Transfer Techniques Genetic Engineering Industrial Microbiology Minerals/metabolism Pseudomonas/genetics,metabolism
Chemicals
Minerals
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ramos J L
Consejo Superior de Investigaciones Científicas-Estación Experimental del Zaidín, Department of Biochemistry and Molecular and Cellular Biology of Plants, Granada, Spain.
Díaz E
Dowling D
de Lorenzo V
Molin S
O'Gara F
Ramos C
Timmis K N
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Article Info
Journal
Bio/technology (Nature Publishing Company)
Abbr.
Biotechnology (N Y)
ISSN
0733-222X
DOI
10.1038/nbt1294-1349
Published
1994-12-00
Pages
1349-56
Language
English
Region
United States
NLM ID
8309273
PMCID
PMC7097320
External Links
PubMed source DOI: 10.1038/nbt1294-1349 PMC Full Text
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