Home LiteratureArticle Details
PMID: 7765117 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A rapid method for measuring organelle-specific substrate transport in homogenates from plant tissues.

Planta ·Vol. 194 ·No. 2 ·1994-00-00 ·Pages 181-5

Flügge UI, Weber A

Abstract

A rapid method is described for measuring organelle-specific metabolite transport systems in crude homogenates from plants. The tissues were homogenized in liquid nitrogen, extracted with buffer and reconstituted into artificial membranes. The method allowed demonstration of the known different substrate specificities of chloroplast triose phosphate/phosphate translocators from C3- and C4-plants, of the triose phosphate/phosphate translocator from non-green tissue, and of the dicarboxylate translocator. It thus by-passes the necessity to isolate intact plant organelles and, in addition, only a low amount of tissue material is required for transport measurements.

MeSH Terms
Biological Transport Carbon Radioisotopes Cell Membrane/metabolism Chloroplasts/metabolism Liposomes Malates/metabolism Organelles/metabolism Phosphates/metabolism Phosphorus Radioisotopes Plants/metabolism Species Specificity Sugar Phosphates/metabolism
Chemicals
Carbon Radioisotopes Liposomes Malates Phosphates Phosphorus Radioisotopes Sugar Phosphates
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Flügge U I
Julius-von-Sachs-Institut für Biowissenschaften, Universität Würzburg, Germany.
Weber A
References (13)
13 references, click to expand
  1. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
    Plant Physiol. 1949 Jan;24(1):1-15 PMID: 16654194
  2. Reconstitution of adenine nucleotide transport with purified ADP, ATP-carrier protein.
    FEBS Lett. 1977 Oct 15;82(2):363-7 PMID: 913611
  3. Molecular aspects of isolated and reconstituted carrier proteins from animal mitochondria.
    Biochim Biophys Acta. 1989 Apr 17;974(1):1-23 PMID: 2647142
  4. Purification and functional reconstitution of the 2-oxoglutarate/malate translocator from spinach chloroplasts.
    Biochim Biophys Acta. 1993 Apr 8;1147(1):13-8 PMID: 8466923
  5. Specific transport of inorganic phosphate, 3-phosphoglycerate and triosephosphates across the inner membrane of the envelope in spinach chloroplasts.
    Biochim Biophys Acta. 1978 May 10;502(2):232-47 PMID: 656403
  6. Cloning and in vivo expression of functional triose phosphate/phosphate translocators from C3- and C4-plants: evidence for the putative participation of specific amino acid residues in the recognition of phosphoenolpyruvate.
    Plant J. 1994 Feb;5(2):215-26 PMID: 8148878
  7. Antisense repression of the chloroplast triose phosphate translocator affects carbon partitioning in transgenic potato plants.
    Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6160-4 PMID: 11607409
  8. Comparison of the kinetic properties, inhibition and labelling of the phosphate translocators from maize and spinach mesophyll chloroplasts.
    Planta. 1990 Jan;180(2):262-71 PMID: 24201955
  9. A Two-Translocator Model for the Transport of 2-Oxoglutarate and Glutamate in Chloroplasts during Ammonia Assimilation in the Light.
    Plant Physiol. 1987 Jul;84(3):624-32 PMID: 16665492
  10. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  11. Reaction mechanism and asymmetric orientation of the reconstituted chloroplast phosphate translocator.
    Biochim Biophys Acta. 1992 Sep 21;1110(1):112-8 PMID: 1390831
  12. Characterization of Glucose-6-Phosphate Incorporation into Starch by Isolated Intact Cauliflower-Bud Plastids.
    Plant Physiol. 1993 Feb;101(2):573-578 PMID: 12231712
  13. Reconstitution and purification of the D-glucose transporter from human erythrocytes.
    J Biol Chem. 1977 Oct 25;252(20):7384-90 PMID: 903365
Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
Published
1994-00-00
Pages
181-5
Language
English
Region
Germany
NLM ID
1250576
External Links
PubMed source View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2