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题目:: Immortalization of human T lymphocytes by oncogenes.
作者:: Okano(F),Tachibana(H),Akiyama(K),Shirahata(S),Murakami(H)
状态:: 发布时间1993-11-16 , 更新时间 2008-10-09
期刊:: Cytotechnology
摘要:: Immortalized human T cell lines were established by cotransfecting c-Ha-ras and c-myc oncogenes to lymph node lymphocytes. The cell lines kept growing for 3 months after establishment without a decrease in growth rate. The cells did not require interleukin-2 (IL-2) for their growth, but addition of IL-2 stimulated the growth of these cells. Flow cytometric analysis revealed that these cells were T cells expressing CD4 or CD8 antigens. A CD4 positive (CD4+) cell line produced IL-6, indicating that the cell line belongs to helper T cells. The CD8 positive (CD8+) cell line possessed cytotoxicity to tumor cells, indicating that the cell line were killer T cells. Both cell lines were able to proliferate in serum-free medium indefinitely.
语言:: eng
DOI:

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