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PMID: 7749916 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Are buried salt bridges important for protein stability and conformational specificity?

Nature structural biology ·Vol. 2 ·No. 2 ·1995-02-00 ·Pages 122-8

Waldburger CD, Schildbach JF, Sauer RT

Abstract

The side chains of Arg 31, Glu 36 and Arg 40 in Arc repressor form a buried salt-bridge triad. The entire salt-bridge network can be replaced by hydrophobic residues in combinatorial randomization experiments resulting in active mutants that are significantly more stable than wild type. The crystal structure of one mutant reveals that the mutant side chains pack against each other in an otherwise wild-type fold. Thus, simple hydrophobic interactions provide more stabilizing energy than the buried salt bridge and confer comparable conformational specificity.

MeSH Terms

Alanine/chemistry Amino Acid Sequence Amino Acids/chemistry Bacteriophage P22/chemistry Circular Dichroism Crystallography, X-Ray DNA, Viral/metabolism Hydrogen Bonding Molecular Sequence Data Mutagenesis, Site-Directed Operator Regions, Genetic Protein Binding Protein Conformation Protein Denaturation Protein Engineering Protein Folding Protein Structure, Secondary Repressor Proteins/chemistry,genetics,metabolism Viral Proteins/chemistry,genetics,metabolism Viral Regulatory and Accessory Proteins

Chemicals

Amino Acids DNA, Viral Repressor Proteins Viral Proteins Viral Regulatory and Accessory Proteins phage repressor proteins Alanine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Waldburger C D

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Schildbach J F

Sauer R T

Article Info

Journal

Nature structural biology

Abbr.

Nat Struct Biol

ISSN

1072-8368

DOI

10.1038/nsb0295-122

Published

1995-02-00

Pages

122-8

Language

English

Region

United States

NLM ID

9421566

Subset

Corrections

CommentIn

PMID: 7749923

External Links

PubMed source DOI: 10.1038/nsb0295-122

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Are buried salt bridges important for protein stability and conformational specificity?

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