An improved PCR method for walking in uncloned genomic DNA.

Nucleic acids research ·Vol. 23 ·No. 6 ·1995-03-25 ·Pages 1087-8

Siebert PD, Chenchik A, Kellogg DE, Lukyanov KA, Lukyanov SA

Abstract

暂无摘要

MeSH Terms

Base Sequence Chromosome Walking/methods Genome Humans Molecular Sequence Data Polymerase Chain Reaction/methods Restriction Mapping Tissue Plasminogen Activator/genetics

Chemicals

Tissue Plasminogen Activator

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Siebert P D

Clontech Laboratories, Inc., Palo Alto, CA 94303-4607, USA.

Chenchik A

Kellogg D E

Lukyanov K A

Lukyanov S A

References (11)

11 references, click to expand

The human tissue plasminogen activator gene.
J Biol Chem. 1986 May 25;261(15):6972-85 PMID: 3009482
Genetic applications of an inverse polymerase chain reaction.
Genetics. 1988 Nov;120(3):621-3 PMID: 2852134
A novel, rapid method for the isolation of terminal sequences from yeast artificial chromosome (YAC) clones.
Nucleic Acids Res. 1990 May 25;18(10):2887-90 PMID: 2161516
Genomic walking and sequencing by oligo-cassette mediated polymerase chain reaction.
Nucleic Acids Res. 1990 May 25;18(10):3095-6 PMID: 2349129
Targeted gene walking polymerase chain reaction.
Nucleic Acids Res. 1991 Jun 11;19(11):3055-60 PMID: 2057362
PCR with end trimming and cassette ligation: a rapid method to clone exon-intron boundaries and a 5'-upstream sequence of genomic DNA based on a cDNA sequence.
PCR Methods Appl. 1994 Aug;4(1):19-25 PMID: 9018312
Genome walking with 2- to 4-kb steps using panhandle PCR.
PCR Methods Appl. 1993 Feb;2(3):197-203 PMID: 7680264
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216-20 PMID: 8134376
Effective amplification of long targets from cloned inserts and human genomic DNA.
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9 PMID: 8202550
Gene walking by unpredictably primed PCR.
Nucleic Acids Res. 1994 Aug 11;22(15):3247-8 PMID: 8065944
Capture PCR: efficient amplification of DNA fragments adjacent to a known sequence in human and YAC DNA.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/23.6.1087

Published

1995-03-25

Pages

1087-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC306810

Subset

External Links

PubMed source DOI: 10.1093/nar/23.6.1087 PMC Full Text View journal details

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An improved PCR method for walking in uncloned genomic DNA.

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