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PMID: 7724534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calcium sensing receptor: molecular cloning in rat and localization to nerve terminals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 8 ·1995-04-11 ·Pages 3161-5

Ruat M, Molliver ME, Snowman AM, Snyder SH

Abstract

We have molecularly cloned a calcium sensing receptor (CaSR) from a rat striatal cDNA library. Rat CaSR displays 92% overall homology to its bovine counterpart with seven putative transmembrane domains characteristic of the superfamily of guanine nucleotide-binding proteins and significant homology with the metabotropic glutamate receptors. Northern blot analysis reveals two transcripts in thyroid, kidney, lung, ileum, and pituitary. In brain highest regional expression of the RNA occurs in the hypothalamus and the corpus striatum. Immunohistochemistry reveals discrete punctate localizations throughout the brain that appear to be associated with nerve terminals. No staining is evident in cell bodies of neurons or glia. Cerebral arteries display an intense network of CaSR immunoreactive fibers associated with vessel innervation. CaSR on nerve terminal membranes may regulate neurotransmitter disposition in response to Ca2+ levels in the synaptic space.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Brain/blood supply Brain Chemistry Calcium/metabolism Cloning, Molecular Corpus Striatum/chemistry DNA, Complementary/genetics Hypothalamus/chemistry Immunoblotting Immunohistochemistry Molecular Sequence Data Nerve Endings/chemistry Nerve Tissue Proteins/genetics,immunology,isolation & purification Protein Conformation Rats Receptors, Calcium-Sensing Receptors, Cell Surface/genetics,immunology,isolation & purification Tissue Distribution
Chemicals
DNA, Complementary Nerve Tissue Proteins Receptors, Calcium-Sensing Receptors, Cell Surface extracellular calcium cation-sensing receptor, rat Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ruat M
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Molliver M E
Snowman A M
Snyder S H
References (25)
25 references, click to expand
  1. Cloning and tissue expression of a rat histamine H2-receptor gene.
    Biochem Biophys Res Commun. 1991 Sep 30;179(3):1470-8 PMID: 1930188
  2. Extracellular Ca2+ sensing, regulation of parathyroid cell function, and role of Ca2+ and other ions as extracellular (first) messengers.
    Physiol Rev. 1991 Apr;71(2):371-411 PMID: 2006218
  3. Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction.
    J Biol Chem. 1992 Jul 5;267(19):13361-8 PMID: 1320017
  4. Possible origins and distribution of immunoreactive nitric oxide synthase-containing nerve fibers in cerebral arteries.
    J Cereb Blood Flow Metab. 1993 Jan;13(1):70-9 PMID: 7678014
  5. Differential localization of phosphoinositide-linked metabotropic glutamate receptor (mGluR1) and the inositol 1,4,5-trisphosphate receptor in rat brain.
    J Neurosci. 1993 May;13(5):2001-12 PMID: 8386753
  6. Molecular characterization of a novel retinal metabotropic glutamate receptor mGluR6 with a high agonist selectivity for L-2-amino-4-phosphonobutyrate.
    J Biol Chem. 1993 Jun 5;268(16):11868-73 PMID: 8389366
  7. The ligand-binding domain in metabotropic glutamate receptors is related to bacterial periplasmic binding proteins.
    Neuron. 1993 Jul;11(1):41-52 PMID: 8338667
  8. Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation.
    Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8547-51 PMID: 8397408
  9. Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid.
    Nature. 1993 Dec 9;366(6455):575-80 PMID: 8255296
  10. Mutations in the human Ca(2+)-sensing receptor gene cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism.
    Cell. 1993 Dec 31;75(7):1297-303 PMID: 7916660
  11. Injection of bovine parathyroid poly(A)+ RNA into Xenopus oocytes confers sensitivity to high extracellular calcium.
    J Bone Miner Res. 1994 Feb;9(2):293-300 PMID: 7511319
  12. Cloning and expression of a new member of the L-2-amino-4-phosphonobutyric acid-sensitive class of metabotropic glutamate receptors.
    Mol Pharmacol. 1994 Mar;45(3):367-72 PMID: 8145723
  13. Endothelial nitric oxide synthase localized to hippocampal pyramidal cells: implications for synaptic plasticity.
    Proc Natl Acad Sci U S A. 1994 May 10;91(10):4214-8 PMID: 7514300
  14. Autosomal dominant hypocalcaemia caused by a Ca(2+)-sensing receptor gene mutation.
    Nat Genet. 1994 Nov;8(3):303-7 PMID: 7874174
  15. Another turn for E-F hands.
    Nat Struct Biol. 1994 Apr;1(4):209-12 PMID: 7656044
  16. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  17. Rapid mobilization of cellular Ca2+ in bovine parathyroid cells evoked by extracellular divalent cations. Evidence for a cell surface calcium receptor.
    J Biol Chem. 1987 Apr 15;262(11):5188-96 PMID: 3558389
  18. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  19. High calcium and other divalent cations increase inositol trisphosphate in bovine parathyroid cells.
    Endocrinology. 1988 Jul;123(1):382-9 PMID: 3260174
  20. Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family.
    Science. 1989 Aug 4;245(4917):494-9 PMID: 2502842
  21. Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain.
    Science. 1989 Aug 4;245(4917):525-8 PMID: 2502844
  22. Bacterial periplasmic binding protein tertiary structures.
    J Biol Chem. 1989 Sep 25;264(27):15739-42 PMID: 2674114
  23. The EF-hand family of calcium-modulated proteins.
    Trends Neurosci. 1989 Nov;12(11):462-7 PMID: 2479149
  24. Sequence and expression of a metabotropic glutamate receptor.
    Nature. 1991 Feb 28;349(6312):760-5 PMID: 1847995
  25. A family of metabotropic glutamate receptors.
    Neuron. 1992 Jan;8(1):169-79 PMID: 1309649
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.8.3161
Published
1995-04-11
Pages
3161-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC42125
Subset
IM
Grants
NIDA NIH HHS · DA-00074 · United States
NIDA NIH HHS · DA-00266 · United States
NIDA NIH HHS · DA-04431 · United States
Databases
GENBANK
U20289
External Links
PubMed source DOI: 10.1073/pnas.92.8.3161 PMC Full Text View journal details
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