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PMID: 770452 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fatty acid transport by the lipophilic bacterium Nocardia asteroides.

Journal of bacteriology ·Vol. 126 ·No. 2 ·1976-05-00 ·Pages 751-7

Calmes R, Deal SJ

Abstract

Hexadecanoate was translocated in Nocardia asteroides by a constitutive transport system(s), which transported short, medium, and long-chain fatty acids. Inhibition of hexadenocanoate transport by homologues suggested that at least two systems are present: one specific for short-chain fatty acids and the other specific for medium- and long-chain fatty acids. Saturation kinetics typical of a carrier-mediated transport system (Kt = 870 muM)were observed, and concentration of fatty acids against a gradient was achieved. Inhibitor studies indicated that free sulfhydryl groups, a functional respiratory chain, and energy are required for translocation. Efflux of [14C]hexadecanoate in the presence of excess unlabeled hexadecanoate or 2,4-dinitrophenol and the cytoplasmic localization of acyl-coenzyme A synthetase (acid:coenzyme A ligase [adenosine monophosphate]; EC 6.2.1.3) (Calmes and Deal, 1973) are consistent with the hypothesis that fatty acids are transported and released intracellularly as free fatty acids.

MeSH Terms
Adsorption Biological Transport, Active Cell Membrane/metabolism Coenzyme A Ligases/metabolism Fatty Acids/metabolism Kinetics Nocardia asteroides/enzymology,metabolism Palmitates/metabolism Sulfhydryl Reagents/pharmacology Uncoupling Agents/pharmacology
Chemicals
Fatty Acids Palmitates Sulfhydryl Reagents Uncoupling Agents Coenzyme A Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Calmes R
Deal S J
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.2.751-757.1976
Published
1976-05-00
Pages
751-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233210
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.2.751-757.1976 PMC Full Text View journal details
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