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PMID: 770440 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Active subunits of Escherichia coli glutamate synthase.

Journal of bacteriology ·Vol. 126 ·No. 1 ·1976-04-00 ·Pages 539-41

Mantsala P, Zalkin H

Abstract

The large and small subunits of Escherichia coli glutamate synthase were isolated. The small subunit catalyzes the NH(3)-dependent synthesis of glutamate. The large subunit exhibits glutaminase activity.

MeSH Terms
Ammonia/metabolism Cell-Free System Escherichia coli/enzymology Glutamate Dehydrogenase/immunology,isolation & purification Glutamate Synthase/immunology,isolation & purification,metabolism Glutamates/biosynthesis Glutaminase/metabolism Glutamine/metabolism Transaminases
Chemicals
Glutamates Glutamine Ammonia Glutamate Synthase Glutamate Dehydrogenase Transaminases Glutaminase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mantsala P
Zalkin H
References (7)
7 references, click to expand
  1. Glutamine-binding subunit of glutamate synthase and partial reactions catalyzed by this glutamine amidotransferase.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4607-11 PMID: 4531004
  2. Reversible denaturation of enzymes by sodium dodecyl sulfate.
    J Biol Chem. 1971 Jul 25;246(14):4504-9 PMID: 5106387
  3. Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein.
    J Biol Chem. 1972 Nov 25;247(22):7407-19 PMID: 4565085
  4. Anthranilate synthetase.
    Adv Enzymol Relat Areas Mol Biol. 1973;38:1-39 PMID: 4275326
  5. Altered alpha subunits in phenylalanyl-tRNA synthetases from p-fluorophenylalanine-resistant strains of Escherichis coli.
    Eur J Biochem. 1975 Jul 1;55(2):431-7 PMID: 1104359
  6. Alternative substrates for glutamate dehydrogenases.
    Biochem Biophys Res Commun. 1975 Jan 6;62(1):78-84 PMID: 234220
  7. A rapid radioactive assay for glutamine synthetase, glutaminase, asparagine synthetase, and asparaginase.
    Anal Biochem. 1970 Oct;37(2):429-38 PMID: 4920285
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.1.539-541.1976
Published
1976-04-00
Pages
539-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233314
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.1.539-541.1976 PMC Full Text View journal details
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