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PMID: 7693718 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tenascin is a cytoadhesive extracellular matrix component of the human hematopoietic microenvironment.

The Journal of cell biology ·Vol. 123 ·No. 4 ·1993-11-00 ·Pages 1027-35

Klein G, Beck S, Müller CA

Abstract

Tenascin is a large extracellular matrix (ECM) glycoprotein found in restricted tissue locations in the adult organism. It is copiously synthesized in regenerative organs or regenerating tissues and by certain tumors. We have analyzed the expression of tenascin in human long term bone marrow cultures as well as in cryostat sections of native bone marrow and found it strongly expressed by the stromal cells of the microenvironment. Two different protein subunits of 280 and 220 kD were detected by immunoblotting. These two forms are derived most likely from two different mRNA splice variants of 6 and 8 kb detected by Northern blotting. The in vivo analysis of cryostat sections showed a codistribution with other ECM molecules such as fibronectin and collagen type III in the microenvironment surrounding the maturing hematopoietic cells. Using two independent cell adhesion assays tenascin could be shown to function as a cytoadhesive molecule for hematopoietic cells. These data suggest a direct involvement of tenascin in the retention of hematopoietic progenitor cells in the stroma.

MeSH Terms
Blotting, Northern Bone Marrow/metabolism Bone Marrow Cells Cell Adhesion Cell Adhesion Molecules, Neuronal/metabolism Cells, Cultured Extracellular Matrix/metabolism Extracellular Matrix Proteins/metabolism Hematopoiesis Humans Immunoblotting Immunohistochemistry RNA Splicing Stromal Cells/metabolism Tenascin
Chemicals
Cell Adhesion Molecules, Neuronal Extracellular Matrix Proteins Tenascin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klein G
University Medical Clinic, Department of Internal Medicine II, Tübingen, Federal Republic of Germany.
Beck S
Müller C A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.123.4.1027
Published
1993-11-00
Pages
1027-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200140
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.123.4.1027 PMC Full Text View journal details
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