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PMID: 7692394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Biophysical characterization of gap-junction channels in HeLa cells.

Pflugers Archiv : European journal of physiology ·Vol. 424 ·No. 3-4 ·1993-08-00 ·Pages 335-42

Eckert R, Dunina-Barkovskaya A, Hülser DF

Abstract

HeLa cells seem not to be junctionally coupled when probed with techniques such as Lucifer yellow spreading and/or ionic coupling measured with three inserted microelectrodes. When investigated with double whole-cell patch-clamp measurements, HeLa cells in monolayer cultures were electrically coupled in 39% of the cases with very low transjunctional conductances (average one to five open channels). These gap-junction channels had a single-channel conductance gamma = 26 +/- 6 pS and were voltage-gated with an equivalent gating charge z = 3.1 +/- 1.5 for a voltage of half-maximal inactivation Uo = 49 +/- 10 mV. The voltage-dependent component represents only 31 +/- 8% of the total junctional conductance. The voltage-insensitive conductance is characterized by a residual open probability po (infinity) = 0.34 +/- 0.12, which corresponds to a ratio Gmin/Gmax = 0.50 +/- 0.12. Dissociation of monolayer cells into cell pairs yielded about 58% coupled cell pairs with no notably altered single-channel properties.

MeSH Terms
Connexins/genetics,metabolism Electrophysiology Gap Junctions/metabolism HeLa Cells Humans Ion Channels/metabolism Isoquinolines Microelectrodes Transfection
Chemicals
Connexins Ion Channels Isoquinolines lucifer yellow
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eckert R
Abteilung Biophysik, Universität Stuttgart, Germany.
Dunina-Barkovskaya A
Hülser D F
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00384361
Published
1993-08-00
Pages
335-42
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00384361 View journal details
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