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PMID: 7688789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ontogeny-related changes in proliferative potential of human hematopoietic cells.

The Journal of experimental medicine ·Vol. 178 ·No. 3 ·1993-09-01 ·Pages 787-91

Lansdorp PM, Dragowska W, Mayani H

Abstract

Blood cells originate from hematopoietic stem cells that are located at different sites during ontogeny. Production of human stem cells and their progeny in culture is expected to have important implications for experimental therapeutic strategies involving gene transfer and transplantation. Here we report striking differences between primitive hematopoietic cells purified from adult bone marrow, umbilical cord blood, and fetal liver in cytokine-supplemented, serum-free cultures. In such cultures both the fraction of responding cells and their ability to produce CD34+ progenitor cells decreased markedly with the age of the cell donor. These results document extensive, ontogeny-related functional differences between primitive hematopoietic cells.

MeSH Terms
Adult Age Factors Antigens, CD/analysis Antigens, CD34 Antigens, Differentiation, B-Lymphocyte/analysis Bone Marrow Cells Cell Division Fetal Blood/cytology Hematopoiesis Hematopoietic Stem Cells/cytology Humans Leukocyte Common Antigens/analysis Liver/embryology Receptors, Transferrin
Chemicals
Antigens, CD Antigens, CD34 Antigens, Differentiation, B-Lymphocyte CD71 antigen Receptors, Transferrin Leukocyte Common Antigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lansdorp P M
Terry Fox Laboratory, University of British Columbia, Vancouver, Canada.
Dragowska W
Mayani H
References (27)
27 references, click to expand
  1. Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells.
    Proc Natl Acad Sci U S A. 1989 May;86(10):3828-32 PMID: 2566997
  2. Human gene therapy comes of age.
    Nature. 1992 Jun 11;357(6378):455-60 PMID: 1608446
  3. Life span of multipotential hematopoietic stem cells in vivo.
    J Exp Med. 1990 May 1;171(5):1407-18 PMID: 2159048
  4. Telomeres shorten during ageing of human fibroblasts.
    Nature. 1990 May 31;345(6274):458-60 PMID: 2342578
  5. Cellular and developmental properties of fetal hematopoietic stem cells.
    Cell. 1990 Jun 15;61(6):953-63 PMID: 1972037
  6. A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells.
    Cell. 1990 Sep 7;62(5):863-74 PMID: 1975515
  7. Expansion in vitro of retrovirally marked totipotent hematopoietic stem cells.
    Blood. 1990 Sep 15;76(6):1071-6 PMID: 2400803
  8. Steel locus defines new multipotent growth factor.
    Cell. 1990 Oct 5;63(1):5-6 PMID: 2208282
  9. The effect of recombinant mast cell growth factor on purified murine hematopoietic stem cells.
    J Exp Med. 1991 May 1;173(5):1205-11 PMID: 1708810
  10. Hematopoietic development of embryonic stem cells in vitro: cytokine and receptor gene expression.
    Genes Dev. 1991 May;5(5):728-40 PMID: 1709130
  11. Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor.
    Blood. 1991 Jun 1;77(11):2316-21 PMID: 1710148
  12. Does stem cell exhaustion result from combining hematopoietic growth factors with chemotherapy? If so, how do we prevent it?
    Blood. 1992 Jul 1;80(1):3-7 PMID: 1377052
  13. Growth of human umbilical-cord blood in longterm haemopoietic cultures.
    Lancet. 1992 Jul 11;340(8811):73-6 PMID: 1352013
  14. The kit receptor and its ligand, steel factor, as regulators of hemopoiesis.
    Cancer Cells. 1991 Dec;3(12):480-7 PMID: 1726456
  15. Ex vivo expansion and maturation of peripheral blood CD34+ cells into the myeloid lineage.
    Blood. 1992 Sep 15;80(6):1405-12 PMID: 1381625
  16. Formation of haematopoietic microenvironment and haematopoietic stem cells from single human bone marrow stem cells.
    Nature. 1992 Dec 24-31;360(6406):745-9 PMID: 1281519
  17. Hematopoietic commitment during embryonic stem cell differentiation in culture.
    Mol Cell Biol. 1993 Jan;13(1):473-86 PMID: 8417345
  18. Enrichment, characterization, and responsiveness of single primitive CD34 human umbilical cord blood hematopoietic progenitors with high proliferative and replating potential.
    Blood. 1993 Jan 1;81(1):41-8 PMID: 7678069
  19. Review: Stratton Lecture 1990. Clinical implications of positive and negative hematopoietic stem cell regulators.
    Blood. 1991 Jul 1;78(1):1-19 PMID: 2070044
  20. Enhancement of murine blast cell colony formation in culture by recombinant rat stem cell factor, ligand for c-kit.
    Blood. 1991 Sep 1;78(5):1223-9 PMID: 1715219
  21. Role of c-kit ligand in the expansion of human hematopoietic progenitor cells.
    Blood. 1992 Feb 1;79(3):634-41 PMID: 1370637
  22. Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation.
    Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1502-6 PMID: 1371359
  23. Preclinical evaluation of human hematolymphoid function in the SCID-hu mouse.
    Immunol Rev. 1991 Dec;124:45-62 PMID: 1804780
  24. Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults.
    Proc Natl Acad Sci U S A. 1992 May 1;89(9):4109-13 PMID: 1373894
  25. Long-term generation of colony-forming cells in liquid culture of CD34+ cord blood cells in the presence of recombinant human stem cell factor.
    Blood. 1992 May 15;79(10):2620-7 PMID: 1375106
  26. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow.
    J Exp Med. 1992 Jun 1;175(6):1501-9 PMID: 1375263
  27. Stable cell membrane labelling.
    Nature. 1989 Jul 13;340(6229):167-8 PMID: 2662017
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.178.3.787
Published
1993-09-01
Pages
787-91
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191172
Subset
IM
Grants
NIAID NIH HHS · AI-29524 · United States
External Links
PubMed source DOI: 10.1084/jem.178.3.787 PMC Full Text View journal details
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