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PMID: 7686210 Published · ppublish English Journal Article

Human B cell precursors proliferate and express CD23 after CD40 ligation.

The Journal of experimental medicine ·Vol. 178 ·No. 1 ·1993-07-01 ·Pages 113-20

Saeland S, Duvert V, Moreau I, Banchereau J

Abstract

The CD40 surface membrane molecule plays an important role in the activation of mature human B cells, but its role in earlier stages of B lineage development is unknown. Here, we have investigated the effects of triggering the CD40 antigen on B cell precursors (BCP) by crosslinking with anti-CD40 antibody presented by Fc gamma-receptor type II-transfected murine Ltk- cells (CD40 system). CD10+ surface immunoglobulin negative (sIg-) BCP, freshly isolated from fetal bone marrow or precultured on stromal cells, proliferated in the CD40 system. This effect required the presence of IL-3, which acted as a specific cosignal among a panel of cytokines examined. The association of IL-10 and IL-7 potentiated the observed IL-3 and CD40-dependent BCP proliferation, demonstrating that IL-10 can act on early B lineage cells. CD40-dependent activation of fetal BCP did not favor maturation to sIg+ B cells, but resulted in the induction of high levels of surface membrane CD23. The emerging CD23+ BCP lacked sIg and CD10, and represented an important proportion of the cycling cells in the CD40-dependent cultures. Taken together, our data demonstrate that stimulation of the CD40 antigen induces expression of the CD23 gene, and regulates cell proliferation during normal human B cell ontogeny.

MeSH Terms
Animals Antigens, CD/physiology Antigens, Differentiation, B-Lymphocyte/physiology B-Lymphocytes/immunology CD40 Antigens Cells, Cultured Female Hematopoietic Stem Cells/immunology Humans Interleukin-3/pharmacology Lymphocyte Activation Mice Neprilysin/analysis Pregnancy Receptors, Complement 3d/analysis Receptors, IgE/analysis
Chemicals
Antigens, CD Antigens, Differentiation, B-Lymphocyte CD40 Antigens Interleukin-3 Receptors, Complement 3d Receptors, IgE Neprilysin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Saeland S
Schering-Plough Laboratory for Immunological Research, Dardilly, France.
Duvert V
Moreau I
Banchereau J
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47 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.178.1.113
Published
1993-07-01
Pages
113-20
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191089
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.178.1.113 PMC Full Text View journal details
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