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PMID: 7681992 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

cDNA for R-cognin: homology with a multifunctional protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 7 ·1993-04-01 ·Pages 2950-4

Krishna Rao AS, Hausman RE

Abstract

Retina cognin (R-cognin) is a developmentally regulated 50-kDa protein that was isolated from chicken embryo retina cell membranes. It mediates the adhesion and reaggregation in vitro of retina cells from chicken and mouse embryos, but not of cells from other tissues, and may be involved in neuronal differentiation. We report here the cloning of a cDNA for R-cognin. A chicken embryo retina cDNA library was constructed in lambda gt11 vector and was screened with polyclonal R-cognin antiserum, yielding several immunoreactive clones. Antiserum prepared to the R-cognin-beta-galactosidase fusion protein produced by one recombinant lysogen recognized the 50-kDa R-cognin protein derived from retina cell membranes. This antiserum inhibited the reaggregation of dissociated retina cells and immunostained chicken embryo retina tissue in a pattern similar to that obtained with R-cognin antiserum. In vitro translation of RNA from a cDNA subclone yielded a 50-kDa protein that was recognized by R-cognin antiserum on a Western blot. By these criteria we identify the cDNA clone as representative of the gene encoding R-cognin. This cDNA is nearly identical to a major portion of the cDNA for the multifunctional protein that is the beta subunit of prolyl 4-hydroxylase and has both protein disulfide isomerase activity and thyroid hormone-binding activity. These findings demonstrate that R-cognin differs from other cell adhesion molecules and suggest possible mechanisms for its action in cell adhesion and neuronal differentiation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Adhesion Cell Aggregation Chick Embryo Chickens Cloning, Molecular DNA/genetics,isolation & purification Escherichia coli/genetics Gene Library Isomerases/genetics Kinetics Membrane Proteins/analysis,genetics,physiology Molecular Sequence Data Plasmids Poly A/genetics,isolation & purification Procollagen-Proline Dioxygenase/genetics Protein Biosynthesis Protein Disulfide-Isomerases RNA/genetics,isolation & purification RNA, Messenger Receptors, Thyroid Hormone/genetics Recombinant Fusion Proteins/analysis,biosynthesis Restriction Mapping Retina/physiology Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transcription, Genetic beta-Galactosidase/analysis,biosynthesis
Chemicals
Membrane Proteins RNA, Messenger Receptors, Thyroid Hormone Recombinant Fusion Proteins cognin Poly A RNA DNA Procollagen-Proline Dioxygenase beta-Galactosidase Isomerases Protein Disulfide-Isomerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krishna Rao A S
Department of Biology, Boston University, MA 02215.
Hausman R E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.7.2950
Published
1993-04-01
Pages
2950-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46214
Subset
IM
Grants
NEI NIH HHS · EY04461 · United States
Databases
GENBANK
L11147
External Links
PubMed source DOI: 10.1073/pnas.90.7.2950 PMC Full Text View journal details
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