Home LiteratureArticle Details
PMID: 7680121 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Replacement of RNA hairpins by in vitro selected tetranucleotides.

Nucleic acids research ·Vol. 21 ·No. 3 ·1993-02-11 ·Pages 531-5

Dichtl B, Pan T, DiRenzo AB, Uhlenbeck OC

Abstract

An in vitro selection method based on the autolytic cleavage of yeast tRNA(Phe) by Pb2+ was applied to obtain tRNA derivatives with the anticodon hairpin replaced by four single-stranded nucleotides. Based on the rates of the site-specific cleavage by Pb2+ and the presence of a specific UV-induced crosslink, certain tetranucleotide sequences allow proper folding of the rest of the tRNA molecule, whereas others do not. One such successful tetramer sequence was also used to replace the acceptor stem of yeast tRNA(Phe) and the anticodon hairpin of E.coli tRNA(Phe) without disrupting folding. These experiments suggest that certain tetramers may be able to replace structurally nonessential hairpins in any RNA.

MeSH Terms
Base Sequence DNA, Single-Stranded Escherichia coli/genetics Lead Molecular Sequence Data Nucleic Acid Conformation Oligoribonucleotides/chemistry RNA, Bacterial/chemistry RNA, Fungal/chemistry RNA, Transfer, Phe/chemistry Saccharomyces cerevisiae/genetics
Chemicals
DNA, Single-Stranded Oligoribonucleotides RNA, Bacterial RNA, Fungal RNA, Transfer, Phe Lead
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dichtl B
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Pan T
DiRenzo A B
Uhlenbeck O C
References (19)
19 references, click to expand
  1. Specific cleavages of pure tRNAs by plumbous ions.
    Biochim Biophys Acta. 1976 May 3;432(2):161-75 PMID: 773433
  2. An ultraviolet light-induced crosslink in yeast tRNA(Phe).
    Nucleic Acids Res. 1992 Aug 11;20(15):4055-9 PMID: 1508690
  3. Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNA.
    Biochemistry. 1985 Aug 27;24(18):4785-801 PMID: 3907691
  4. Comparative anatomy of 16-S-like ribosomal RNA.
    Prog Nucleic Acid Res Mol Biol. 1985;32:155-216 PMID: 3911275
  5. Deletion of nonconserved helices near the 3' end of the rRNA intron of Tetrahymena thermophila alters self-splicing but not core catalytic activity.
    Genes Dev. 1988 Jun;2(6):652-63 PMID: 3417146
  6. Characterization of the lead(II)-induced cleavages in tRNAs in solution and effect of the Y-base removal in yeast tRNAPhe.
    Biochemistry. 1988 Jul 26;27(15):5771-7 PMID: 3179275
  7. Characterization of RNA hairpin loop stability.
    Nucleic Acids Res. 1988 Dec 23;16(24):11725-35 PMID: 3211748
  8. Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.
    Science. 1989 Mar 10;243(4896):1363-6 PMID: 2646717
  9. The design and catalytic properties of a simplified ribonuclease P RNA.
    Science. 1989 Jun 30;244(4912):1569-71 PMID: 2472671
  10. Miniribozymes, small derivatives of the sunY intron, are catalytically active.
    Mol Cell Biol. 1989 Dec;9(12):5480-3 PMID: 2685567
  11. Synthesis of small RNAs using T7 RNA polymerase.
    Methods Enzymol. 1989;180:51-62 PMID: 2482430
  12. Lead ion binding and RNA chain hydrolysis in phenylalanine tRNA.
    J Biomol Struct Dyn. 1983 Dec;1(3):639-46 PMID: 6400892
  13. Lead-catalyzed cleavage of yeast tRNAPhe mutants.
    Biochemistry. 1990 Mar 13;29(10):2515-23 PMID: 2334679
  14. Role of the tertiary nucleotides in the interaction of yeast phenylalanine tRNA with its cognate synthetase.
    Biochemistry. 1990 Mar 13;29(10):2523-32 PMID: 2334680
  15. Self-splicing of group I introns.
    Annu Rev Biochem. 1990;59:543-68 PMID: 2197983
  16. SRP-RNA sequence alignment and secondary structure.
    Nucleic Acids Res. 1991 Jan 25;19(2):209-15 PMID: 1707519
  17. In vitro selection of RNAs that undergo autolytic cleavage with Pb2+.
    Biochemistry. 1992 Apr 28;31(16):3887-95 PMID: 1373649
  18. Minimal sequence requirements for ribozyme activity.
    Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5710-4 PMID: 1631050
  19. Structural domains of transfer RNA molecules.
    Science. 1976 Nov 19;194(4267):796-806 PMID: 790568
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.3.531
Published
1993-02-11
Pages
531-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309149
Subset
IM
Grants
NIGMS NIH HHS · GM 37552 · United States
External Links
PubMed source DOI: 10.1093/nar/21.3.531 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2