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PMID: 7667088 Published · ppublish English Journal Article

Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.

Nucleic acids research ·Vol. 23 ·No. 16 ·1995-08-25 ·Pages 3126-31

Tang K, Fu D, Kötter S, Cotter RJ, Cantor CR, Köster H

Abstract

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

MeSH Terms
Base Sequence DNA Probes/chemistry,genetics Mass Spectrometry/methods Molecular Biology Molecular Sequence Data Nucleic Acid Denaturation Nucleic Acid Hybridization Sequence Analysis, DNA/methods
Chemicals
DNA Probes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tang K
Sequenom Inc., Boston, MA 02110, USA.
Fu D
Kötter S
Cotter R J
Cantor C R
Köster H
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19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.16.3126
Published
1995-08-25
Pages
3126-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC307169
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.16.3126 PMC Full Text View journal details
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