Isolation of rec- mutants from an F-prime merodiploid strain of Escherichia coli K-12.

Molecular & general genetics : MGG ·Vol. 143 ·No. 2 ·1976-01-16 ·Pages 223-32

Stacey KA, Lloyd RG

Abstract

This paper describes a method of screening mutagenised populations of an E. coli galA/galB F- prime merodiploid for mutants defective in recombination. The method relies on scoring colonies on Eosin-Methylene Blue agar that have fewer than normal numbers of Gal+ papillae. With a suitable choice of gal- mutations most of the papillae arise by recombination and some of those colonies with less than normal numbers prove to be defective in some aspect of recombination or DNA repair. In addition to strains carrying mutations that can be ascribed to known loci, several novel mutant phenotypes were identified.

MeSH Terms

Coliphages DNA Repair Escherichia coli Genes Mutation Phenotype Recombination, Genetic Transduction, Genetic

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stacey K A

Lloyd R G

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25 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00266925

Published

1976-01-16

Pages

223-32

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00266925

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Isolation of rec- mutants from an F-prime merodiploid strain of Escherichia coli K-12.

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