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PMID: 7654175 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An upstream stimulatory factor (USF) binding motif is critical for rat preprotachykinin-A promoter activity in PC12 cells.

The Biochemical journal ·Vol. 310 ( Pt 2) ·1995-09-01 ·Pages 401-6

Paterson JM, Morrison CF, Mendelson SC, McAllister J, Quinn JP

Abstract

We demonstrate the presence of a functional E box motif in the proximal rat preprotachykinin-A (rPPT) promoter. This element (spanning nucleotides -67 to -47) exhibits the sequence 5'-CACGTG-3' which is recognized and bound by the basic helix-loop-helix family of regulatory proteins. We also show that at least one of the factors bound to this rPPT promoter element in both HeLa and PC12 nuclear extract is the ubiquitously expressed transcription factor, the upstream stimulatory factor (USF). Mutation of this element by insertion of a 10 bp linker into the E box motif, in an rPPT promoter fragment spanning -865 to +92, destroys the ability of this promoter fragment to support reporter gene expression in a PC12 cell model of rPPT promoter activity. The data indicate that this rPPT E box element is likely to function as an important cis-regulatory domain in the rPPT promoter.

MeSH Terms
Animals Base Sequence Binding Sites Cell Division Cell Nucleus/metabolism DNA-Binding Proteins Electroporation HeLa Cells Helix-Loop-Helix Motifs Humans Molecular Sequence Data Oligonucleotide Probes PC12 Cells Promoter Regions, Genetic Protein Precursors/genetics Rats Regulatory Sequences, Nucleic Acid Tachykinins/genetics Transcription Factors/isolation & purification,metabolism Upstream Stimulatory Factors
Chemicals
DNA-Binding Proteins Oligonucleotide Probes Protein Precursors Tachykinins Transcription Factors USF1 protein, human Upstream Stimulatory Factors Usf1 protein, rat preprotachykinin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Paterson J M
MRC Brain Metabolism Unit, Royal Edinburgh Hospital, Scotland, U.K.
Morrison C F
Mendelson S C
McAllister J
Quinn J P
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3100401
Published
1995-09-01
Pages
401-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135909
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3100401 PMC Full Text View journal details
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