Home LiteratureArticle Details

PMID: 7649300 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.

FEBS letters ·Vol. 370 ·No. 1-2 ·1995-08-14 ·Pages 32-6

McCormack K, McCormack T, Tanouye M, Rudy B, Stühmer W

Abstract

Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferret beta 3 proteins are identical with rat beta 1 throughout the core while beta 2 proteins are not; beta 2 also contains a shorter N-terminus and has no reported physiological role. We report that human beta 1 and beta 3 are derived from the same gene and that beta 2 modulates the inactivation properties of Kv1.4 alpha subunits.

MeSH Terms

Alternative Splicing Amino Acid Sequence Animals Base Sequence Brain/metabolism Cattle Conserved Sequence DNA Primers Female Ferrets Gene Expression Hominidae/genetics Humans Macromolecular Substances Mammals Membrane Potentials/physiology Molecular Sequence Data Myocardium/metabolism Oocytes/physiology Peptide Biosynthesis Peptides/genetics Polymerase Chain Reaction Rats Sequence Homology, Amino Acid Shaker Superfamily of Potassium Channels Transfection Xenopus laevis

Chemicals

DNA Primers Macromolecular Substances Peptides Shaker B potassium channel polypeptide Shaker Superfamily of Potassium Channels

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

McCormack K

Max-Planck-Institüt für experimentelle Medizin, Abteilung 11, Göttingen, Germany.

McCormack T

Tanouye M

Rudy B

Stühmer W

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(95)00785-8

Published

1995-08-14

Pages

32-6

Language

English

Region

England

NLM ID

0155157

Subset

Grants

PHS HHS · IBN9209523 · United States

NINDS NIH HHS · NS30989 · United States

Databases

GENBANK

U33428, U33429

External Links

PubMed source DOI: 10.1016/0014-5793(95)00785-8 View journal details

Analysis Services

Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.

Contact

WeChat Official Account