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PMID: 7647795 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.

Nature genetics ·Vol. 10 ·No. 1 ·1995-05-00 ·Pages 77-83

Hirotsune S, Takahara T, Sasaki N, Hirose K, Yoshiki A, Ohashi T, Kusakabe M, Murakami Y, Muramatsu M, Watanabe S

Abstract

We have identified a strong candidate cDNA for the mouse reeler gene. This 5 kb transcript encodes a 99.4 kD protein consisting of 881 amino acids and possessing two EGF-like motifs. We assayed two independent mutant alleles--'Jackson reeler', which has a deletion of the entire gene, and 'Orleans reeler' which exhibits a 220 bp deletion in the open reading frame, including the second EGF-like motif and resulting in a frame shift. In situ hybridization reveals that the transcript is detected exclusively in the pioneer neurons which guide neuronal cell migration along the radial array. Our findings offer an explanation for how the reeler mutant phenotype causes a disturbance of the complex architecture of the neuronal network.

MeSH Terms

Amino Acid Sequence Animals Animals, Newborn Base Sequence Brain/metabolism Cell Adhesion Molecules, Neuronal/biosynthesis,genetics Chromosome Mapping Cloning, Molecular Conserved Sequence Crosses, Genetic DNA, Complementary Epidermal Growth Factor/genetics Extracellular Matrix Proteins/biosynthesis,genetics Gene Deletion Gene Expression Regulation, Developmental In Situ Hybridization Mice Mice, Inbred BALB C Mice, Neurologic Mutants/genetics Molecular Sequence Data Nerve Tissue Proteins Neurons/metabolism Organ Specificity Reelin Protein Sequence Deletion Serine Endopeptidases

Chemicals

Cell Adhesion Molecules, Neuronal DNA, Complementary Extracellular Matrix Proteins Nerve Tissue Proteins Reelin Protein Epidermal Growth Factor Serine Endopeptidases

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Hirotsune S

Genome Science Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.

Takahara T

Sasaki N

Hirose K

Yoshiki A

Ohashi T

Kusakabe M

Murakami Y

Muramatsu M

Watanabe S

Article Info

Journal

Nature genetics

Abbr.

Nat Genet

ISSN

1061-4036

DOI

10.1038/ng0595-77

Published

1995-05-00

Pages

77-83

Language

English

Region

United States

NLM ID

9216904

Subset

Databases

GENBANK

D63520

Corrections

CommentIn

PMID: 7550305

External Links

PubMed source DOI: 10.1038/ng0595-77 View journal details

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The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.

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