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PMID: 7644517 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The algT (algU) gene of Pseudomonas aeruginosa, a key regulator involved in alginate biosynthesis, encodes an alternative sigma factor (sigma E).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 17 ·1995-08-15 ·Pages 7941-5

Hershberger CD, Ye RW, Parsek MR, Xie ZD, Chakrabarty AM

Abstract

Chronic infection by alginate-producing (mucoid) Pseudomonas aeruginosa is the leading cause of mortality among cystic fibrosis (CF) patients. During the course of sustained infection, the production of an alginate capsule protects the bacteria and allows them to persist in the CF lung. One of the key regulators of alginate synthesis is the algT (algU) gene encoding a putative alternative sigma factor (sigma E). AlgT was hyperproduced and purified from Escherichia coli. The N-terminal sequence of the purified protein matched perfectly with that predicted from the DNA sequence. The purified protein, in the presence of E. coli RNA polymerase core enzyme, was able to initiate transcription of an algT promoter. Deletion of the -35 region of this promoter abolished this activity in vitro as well as in vivo. These data indicate that the algT gene encodes a sigma factor that is autoregulatory.

Related Genes
algC algD algR1 algT algU carQRS dagAP2 rpoHP3
MeSH Terms
Alginates/metabolism Bacterial Proteins/biosynthesis,genetics,isolation & purification Base Sequence Binding Sites DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Gene Expression Gene Expression Regulation, Bacterial Genes, Bacterial Glucuronic Acid Hexuronic Acids Molecular Sequence Data Promoter Regions, Genetic Pseudomonas aeruginosa/genetics,metabolism Recombinant Proteins/biosynthesis,isolation & purification Sequence Homology, Nucleic Acid Sigma Factor/biosynthesis Transcription Factors Transcription, Genetic
Chemicals
AlgU protein, Pseudomonas aeruginosa Alginates Bacterial Proteins Hexuronic Acids Recombinant Proteins Sigma Factor Transcription Factors sporulation-specific sigma factors Glucuronic Acid DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hershberger C D
Department of Microbiology and Immunology (M/C 790, University of Illinois College of Medicine, Chicago 60612, USA.
Ye R W
Parsek M R
Xie Z D
Chakrabarty A M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.17.7941
Published
1995-08-15
Pages
7941-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41262
Subset
IM
Grants
NIAID NIH HHS · AI 16790-14 · United States
NIAID NIH HHS · AI 31546-02 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.17.7941 PMC Full Text View journal details
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