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PMID: 7644475 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 17 ·1995-08-15 ·Pages 7672-6

Lazar G, Schaal T, Maniatis T, Goodman HM

Abstract

We show that the higher plant Arabidopsis thaliana has a serine-arginine-rich (SR) protein family whose members contain a phosphoepitope shared by the animal SR family of splicing factors. In addition, we report the cloning and characterization of a cDNA encoding a higher-plant SR protein from Arabidopsis, SR1, which has striking sequence and structural homology to the human splicing factor SF2/ASF. Similar to SF2/ASF, the plant SR1 protein promotes splice site switching in mammalian nuclear extracts. A novel feature of the Arabidopsis SR protein is a C-terminal domain containing a high concentration of proline, serine, and lysine residues (PSK domain), a composition reminiscent of histones. This domain includes a putative phosphorylation site for the mitotic kinase cyclin/p34cdc2.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/metabolism Arabidopsis Proteins Arginine Base Sequence Cloning, Molecular DNA, Complementary Exons Humans Lysine Mammals Molecular Sequence Data Nuclear Proteins/biosynthesis,chemistry,genetics Plant Proteins/biosynthesis,chemistry,genetics Proline RNA-Binding Proteins Recombinant Proteins/biosynthesis,chemistry Sequence Homology, Amino Acid Serine Serine-Arginine Splicing Factors Spliceosomes/metabolism
Chemicals
Arabidopsis Proteins DNA, Complementary Nuclear Proteins Plant Proteins RNA-Binding Proteins Recombinant Proteins SR1 protein, Arabidopsis Serine-Arginine Splicing Factors Serine Arginine Proline Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lazar G
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
Schaal T
Maniatis T
Goodman H M
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.17.7672
Published
1995-08-15
Pages
7672-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41207
Subset
IM
Databases
GENBANK
M98340
External Links
PubMed source DOI: 10.1073/pnas.92.17.7672 PMC Full Text View journal details
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