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PMID: 7612821 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct behavior of connexin56 and connexin46 gap junctional channels can be predicted from the behavior of their hemi-gap-junctional channels.

Biophysical journal ·Vol. 68 ·No. 5 ·1995-05-00 ·Pages 1796-803

Ebihara L, Berthoud VM, Beyer EC

Abstract

The gap-junctional protein rat connexin46 (Cx46) has the unusual ability to form voltage-gated channels in the nonjunctional plasma membrane of Xenopus oocytes (Paul et al., 1991; Ebihara and Steiner, 1993). These have been suggested to be gap-junctional hemichannels or connexons. The Xenopus oocyte system was used to characterize the functional properties of a closely related lens gap-junctional protein, chicken connexin56 (Cx56) (Rup et al., 1993) and to contrast them to those of rat Cx46. Single oocytes injected with either Cx56 or Cx46 cRNA developed time-dependent, outward currents that activated on depolarization. The currents induced by Cx56 and Cx46 showed differences in steady-state voltage dependence and in their degree of rectification. Furthermore, the voltage-dependent properties of the nonjunctional channels induced by the connexin cRNAs in external solutions containing low concentrations of calcium ions could account remarkably well for the behavior of the intercellular channels formed by Cx56 and Cx46 in paired oocytes. These results suggest that many of the voltage-dependent properties of the hemi-gap-junctional channels are retained by the intercellular channels.

MeSH Terms
Animals Chickens Connexins/biosynthesis,isolation & purification,physiology Electrophysiology/methods Eye Proteins/biosynthesis,isolation & purification,physiology Female Gap Junctions/physiology Immunoblotting Membrane Potentials Microinjections Oocytes/physiology Patch-Clamp Techniques Protein Biosynthesis RNA, Complementary/administration & dosage,metabolism Rats Recombinant Proteins/biosynthesis,isolation & purification,metabolism Time Factors Transcription, Genetic Xenopus
Chemicals
Connexins Eye Proteins RNA, Complementary Recombinant Proteins connexin 46 connexin 56
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ebihara L
Department of Pharmacology, Columbia University, New York, New York 10032, USA.
Berthoud V M
Beyer E C
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30 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(95)80356-5
Published
1995-05-00
Pages
1796-803
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1282082
Subset
IM
Grants
NEI NIH HHS · R01 EY008368 · United States
NEI NIH HHS · EY08368 · United States
NEI NIH HHS · EY10589 · United States
NHLBI NIH HHS · HL45377 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(95)80356-5 PMC Full Text View journal details
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