The expression of nuclear genes encoding plastid ribosomal proteins precedes the expression of chloroplast genes during early phases of chloroplast development.

Plant physiology ·Vol. 108 ·No. 2 ·1995-06-00 ·Pages 685-92

Harrak H, Lagrange T, Bisanz-Seyer C, Lerbs-Mache S, Mache R

Abstract

The development of different plant organs (root, hypocotyl, and cotyledons) during seed germination is connected with the transformation of proplastids, which are found in embryonic and meristematic tissues, into amyloplasts in root tissues and into chloroplasts in cotyledons. We have analyzed the expression of nuclear and plastid genes coding for the plastid translational apparatus during the first 7 d of Spinacia oleracea development. Results show that the nuclear genes (rps1, rps22, rpI21, and rpI40) are expressed from the 1st d of seed imbibition and precede transcription of the chloroplast-encoded genes (photosynthetic and nonphotosynthetic), which starts the 3rd d after the beginning of imbibition. Transcription from the leaf-/cotyledon-specific P1 promoter of the rpI21 gene starts on the first imbibition day. Inhibition of chloroplast biogenesis by bleaching in the presence of norflurazon has no influence on the expression from this P1 promoter, suggesting that the onset of transcription of nuclear gene rpI21 is independent of a plastid signal.

MeSH Terms

Base Sequence Cell Nucleus/metabolism Chloroplasts/metabolism,physiology DNA Primers Gene Expression Regulation, Plant/drug effects Genes, Plant Herbicides/pharmacology Molecular Sequence Data Plastids/metabolism Polymerase Chain Reaction Pyridazines/pharmacology Ribosomal Proteins/biosynthesis Species Specificity Spinacia oleracea/growth & development,metabolism

Chemicals

DNA Primers Herbicides Pyridazines Ribosomal Proteins norflurazone

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Harrak H

Laboratoire de Biologie Moléculaire Végétale, Université J. Fourier, Grenoble, France.

Lagrange T

Bisanz-Seyer C

Lerbs-Mache S

Mache R

References (21)

21 references, click to expand

Proteins encoded by a complex chloroplast transcription unit are each translated from both monocistronic and polycistronic mRNAs.
EMBO J. 1988 Sep;7(9):2637-44 PMID: 2460341
Sequence of Lhcb3*1, a gene encoding a Photosystem II chlorophyll a/b-binding protein in Pisum.
Biochim Biophys Acta. 1993 Jun 25;1173(3):333-6 PMID: 8318543
Structure and transcription of the spinach chloroplast rDNA leader region.
Nucleic Acids Res. 1982 Nov 11;10(21):6865-78 PMID: 6294618
Microspectrofluorometric Measurement of Chloroplast DNA in Dividing and Expanding Leaf Cells of Spinacia oleracea.
Plant Physiol. 1986 Jun;81(2):708-10 PMID: 16664887
Spinach plastid genes coding for initiation factor IF-1, ribosomal protein S11 and RNA polymerase alpha-subunit.
Nucleic Acids Res. 1986 Jan 24;14(2):1029-44 PMID: 3003688
The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA.
Nucleic Acids Res. 1981 Jul 24;9(14):3251-70 PMID: 6269077
The 110-kDa polypeptide of spinach plastid DNA-dependent RNA polymerase: single-subunit enzyme or catalytic core of multimeric enzyme complexes?
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5509-13 PMID: 8516293
Regulation of the synthesis of ribosomes and ribosomal components.
Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
Hypothesis for the evolutionary origin of the chloroplast ribosomal protein L21 of spinach.
Curr Genet. 1990 Dec;18(6):553-6 PMID: 2076556
Plastid transcription activity and DNA copy number increase early in barley chloroplast development.
Plant Physiol. 1989 Mar;89(3):1011-8 PMID: 16666609
Differential expression of genes in potato tubers after wounding.
Proc Natl Acad Sci U S A. 1988 Feb;85(4):1136-40 PMID: 16593908
Signal transduction mutants of Arabidopsis uncouple nuclear CAB and RBCS gene expression from chloroplast development.
Cell. 1993 Sep 10;74(5):787-99 PMID: 7690685
Chloroplast transcription is required to express the nuclear genes rbcS and cab. Plastid DNA copy number is regulated independently.
Plant Mol Biol. 1991 Oct;17(4):813-23 PMID: 1912500
Multicopy GTPase center protein L12 of Arabidopsis chloroplast ribosome is encoded by a clustered nuclear gene family with the expressed members closely linked to tRNA(Pro) genes.
J Biol Chem. 1994 Mar 11;269(10):7330-6 PMID: 8125949
Plastid Genes Encoding the Transcription/Translation Apparatus Are Differentially Transcribed Early in Barley (Hordeum vulgare) Chloroplast Development (Evidence for Selective Stabilization of psbA mRNA).
Plant Physiol. 1993 Mar;101(3):781-791 PMID: 12231729
Chloroplast gene expression: how plants turn their plastids on.
Cell. 1989 Jan 27;56(2):161-70 PMID: 2643471
Dynamic regulation of chloroplast transcription.
Plant Physiol. 1993 Oct;103(2):309-13 PMID: 8029331
Conservation and evolution of the nucleus-encoded and chloroplast-specific ribosomal proteins in pea and spinach.
Gene. 1991 Jul 22;103(2):139-45 PMID: 1889743
Structure and expression of the nuclear gene coding for the plastid CS1 ribosomal protein from spinach.
Nucleic Acids Res. 1992 Aug 25;20(16):4153-7 PMID: 1508710
Effects of induction of rRNA overproduction on ribosomal protein synthesis and ribosome subunit assembly in Escherichia coli.
J Bacteriol. 1988 Nov;170(11):5042-50 PMID: 3053641
Constitutive transcription and regulation of gene expression in non-photosynthetic plastids of higher plants.
EMBO J. 1988 Nov;7(11):3301-8 PMID: 16453858

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.108.2.685

Published

1995-06-00

Pages

685-92

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC157389

Subset

External Links

PubMed source DOI: 10.1104/pp.108.2.685 PMC Full Text View journal details

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The expression of nuclear genes encoding plastid ribosomal proteins precedes the expression of chloroplast genes during early phases of chloroplast development.

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