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PMID: 7609089 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and mapping of inhibitory sequences in the human immunodeficiency virus type 2 vif gene.

Journal of virology ·Vol. 69 ·No. 8 ·1995-08-00 ·Pages 5167-70

Reddy TR, Kraus G, Suhasini M, Leavitt MC, Wong-Staal F

Abstract

Human immunodeficiency virus (HIV) regulates the expression of its genes temporally at the mRNA processing step. A subset of the mRNA species which encode the structural and some accessory genes contains inhibitory sequences (INS or CRS elements) which prevent nuclear export of the RNA or its utilization in the cytoplasm. Such inhibition is overridden by the interaction of a viral protein, Rev, with its RNA target sequence, RRE. The vif gene product, which is essential for virus replication in vivo, is encoded by a singly spliced mRNA, and its expression is dependent on rev in infected cells. However, INS elements have not been found in the HIV-1 vif gene itself, although such elements have been observed in Gag, Pol, and Env coding sequences. We have now identified an INS within the 5' half of HIV-2 vif which does not show any homology with cellular mRNAs or other previously identified INS and CRS elements of HIV. These results suggest that retroviral mRNAs have novel labile sequences different from those of cellular mRNAs.

Related Genes
vif
MeSH Terms
Base Sequence Chloramphenicol O-Acetyltransferase/genetics Defective Viruses/genetics Gene Expression Regulation, Viral Genes, vif HIV-1/genetics HIV-2/genetics HeLa Cells Humans Molecular Sequence Data Oligodeoxyribonucleotides Regulatory Sequences, Nucleic Acid
Chemicals
Oligodeoxyribonucleotides Chloramphenicol O-Acetyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reddy T R
Department of Medicine, University of California, San Diego, La Jolla 92093-0665, USA.
Kraus G
Suhasini M
Leavitt M C
Wong-Staal F
References (43)
43 references, click to expand
  1. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus.
    Nature. 1985 Feb 7-13;313(6002):450-8 PMID: 2982104
  2. A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.
    Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1256-60 PMID: 8108397
  3. Truncation of exon 1 from the c-myc gene results in prolonged c-myc mRNa stability.
    EMBO J. 1985 Dec 30;4(13B):3727-33 PMID: 4092694
  4. Identification of HTLV-III/LAV sor gene product and detection of antibodies in human sera.
    Science. 1986 Mar 28;231(4745):1553-5 PMID: 3006245
  5. Three novel genes of human T-lymphotropic virus type III: immune reactivity of their products with sera from acquired immune deficiency syndrome patients.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2209-13 PMID: 3008154
  6. A second post-transcriptional trans-activator gene required for HTLV-III replication.
    Nature. 1986 May 22-28;321(6068):412-7 PMID: 3012355
  7. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  8. HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.
    Cell. 1986 Sep 12;46(6):807-17 PMID: 3638988
  9. The HIV 'A' (sor) gene product is essential for virus infectivity.
    Nature. 1987 Aug 20-26;328(6132):728-30 PMID: 2441266
  10. The sor gene of HIV-1 is required for efficient virus transmission in vitro.
    Science. 1987 Aug 21;237(4817):888-93 PMID: 3497453
  11. Rapid cytoplasmic turnover of c-myc mRNA: requirement of the 3' untranslated sequences.
    Mol Cell Biol. 1987 Dec;7(12):4513-21 PMID: 3325826
  12. Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
    Nature. 1988 Nov 24;336(6197):396-9 PMID: 3194021
  13. Cis-acting sequences responsive to the rev gene product of the human immunodeficiency virus.
    J Acquir Immune Defic Syndr. 1988;1(5):441-52 PMID: 2851651
  14. The rev (trs/art) protein of human immunodeficiency virus type 1 affects viral mRNA and protein expression via a cis-acting sequence in the env region.
    J Virol. 1989 Mar;63(3):1265-74 PMID: 2783738
  15. The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.
    Nature. 1989 Mar 16;338(6212):254-7 PMID: 2784194
  16. rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1495-9 PMID: 2784208
  17. Regulation of human immunodeficiency virus env expression by the rev gene product.
    J Virol. 1989 May;63(5):1959-66 PMID: 2704072
  18. The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
    Genes Dev. 1989 Jan;3(1):60-72 PMID: 2496006
  19. The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization.
    Cell. 1989 Jun 30;57(7):1155-65 PMID: 2736624
  20. Temporal aspects of DNA and RNA synthesis during human immunodeficiency virus infection: evidence for differential gene expression.
    J Virol. 1989 Sep;63(9):3708-13 PMID: 2760980
  21. Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1.
    J Virol. 1990 Jun;64(6):2519-29 PMID: 2335812
  22. Structure and expression of tat-, rev-, and nef-specific transcripts of human immunodeficiency virus type 1 in infected lymphocytes and macrophages.
    J Virol. 1990 Jul;64(7):3391-8 PMID: 2191150
  23. Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein.
    J Virol. 1990 Aug;64(8):3734-41 PMID: 2196381
  24. Env and Vpu proteins of human immunodeficiency virus type 1 are produced from multiple bicistronic mRNAs.
    J Virol. 1990 Nov;64(11):5448-56 PMID: 2214021
  25. Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
    Genes Dev. 1991 Feb;5(2):221-31 PMID: 1899842
  26. The protein-coding region of c-myc mRNA contains a sequence that specifies rapid mRNA turnover and induction by protein synthesis inhibitors.
    Genes Dev. 1991 Feb;5(2):232-43 PMID: 1995415
  27. A specific inhibitor of cysteine proteases impairs a Vif-dependent modification of human immunodeficiency virus type 1 Env protein.
    J Virol. 1991 Mar;65(3):1325-31 PMID: 1995946
  28. Rev activates expression of the human immunodeficiency virus type 1 vif and vpr gene products.
    J Virol. 1991 Mar;65(3):1653-7 PMID: 1825343
  29. Expression of human immunodeficiency virus type 1 vif and vpr mRNAs is Rev-dependent and regulated by splicing.
    Virology. 1991 Aug;183(2):677-86 PMID: 1830183
  30. The biochemistry of AIDS.
    Annu Rev Biochem. 1991;60:577-630 PMID: 1883204
  31. Identification of posttranscriptionally active inhibitory sequences in human immunodeficiency virus type 1 RNA: novel level of gene regulation.
    J Virol. 1991 Nov;65(11):5732-43 PMID: 1656066
  32. Distinct RNA sequences in the gag region of human immunodeficiency virus type 1 decrease RNA stability and inhibit expression in the absence of Rev protein.
    J Virol. 1992 Jan;66(1):150-9 PMID: 1727477
  33. Elements distinct from human immunodeficiency virus type 1 splice sites are responsible for the Rev dependence of env mRNA.
    J Virol. 1994 May;68(5):2986-93 PMID: 8151769
  34. A cis-acting repressive sequence that overlaps the Rev-responsive element of human immunodeficiency virus type 1 regulates nuclear retention of env mRNAs independently of known splice signals.
    Proc Natl Acad Sci U S A. 1994 Aug 30;91(18):8314-8 PMID: 8078879
  35. Comparative analyses of human immunodeficiency virus type 1 (HIV-1) and HIV-2 Vif mutants.
    J Virol. 1995 Jun;69(6):3549-53 PMID: 7745702
  36. An improved CAT assay for promoter analysis in either transgenic mice or tissue culture cells.
    DNA Cell Biol. 1992 Jan-Feb;11(1):83-90 PMID: 1739436
  37. Cell-free transmission of Vif mutants of HIV-1.
    Virology. 1992 Sep;190(1):19-29 PMID: 1529528
  38. Role of vif in replication of human immunodeficiency virus type 1 in CD4+ T lymphocytes.
    J Virol. 1992 Nov;66(11):6489-95 PMID: 1357189
  39. Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles.
    J Virol. 1993 Mar;67(3):1663-6 PMID: 8437236
  40. Transcomplementation of VIF- HIV-1 mutants in CEM cells suggests that VIF affects late steps of the viral life cycle.
    Virology. 1993 Mar;193(1):186-92 PMID: 8438565
  41. Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
    J Virol. 1993 Aug;67(8):4945-55 PMID: 8331734
  42. The human immunodeficiency virus type 1 (HIV-1) vif protein is located in the cytoplasm of infected cells and its effect on viral replication is equivalent in HIV-2.
    AIDS Res Hum Retroviruses. 1993 Oct;9(10):1025-30 PMID: 8280477
  43. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.8.5167-5170.1995
Published
1995-08-00
Pages
5167-70
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189340
Subset
IM
Grants
NIAID NIH HHS · 1P30-AI-36214-01 · United States
NIAID NIH HHS · AI29889-03 · United States
NIAID NIH HHS · AI30238-03 · United States
External Links
PubMed source DOI: 10.1128/JVI.69.8.5167-5170.1995 PMC Full Text View journal details
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