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PMID: 7601832 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A single amino acid substitution in sigma E affects its ability to bind core RNA polymerase.

Journal of bacteriology ·Vol. 177 ·No. 13 ·1995-07-00 ·Pages 3687-94

Shuler MF, Tatti KM, Wade KH, Moran CP

Abstract

We have examined the role of the most highly conserved region of bacterial RNA polymerase sigma factors by analyzing the effect of amino acid substitutions and small deletions in sigma E from Bacillus subtilis. sigma E is required for the production of endospores in B. subtilis but not for vegetative growth. Strains expressing each of several mutant forms of sigE were found to be deficient in their ability to form endospores. Single amino acid substitutions at positions 68 and 94 resulted in sigma factors that bind with less affinity to the core subunits of RNA polymerase. The substitution at position 68 did not affect the stability of the protein in B. subtilis; therefore, this substitution probably did not have large effects on the overall structure of the sigma factor. The substitution at position 68 probably defines a position in sigma E that closely contacts a subunit of RNA polymerase, while the substitution at position 94 may define a position that is important for protein stability or for binding to core RNA polymerase.

Related Genes
MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics,growth & development Bacterial Proteins/genetics,metabolism Base Sequence Conserved Sequence DNA Mutational Analysis DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology,metabolism Molecular Sequence Data Mutagenesis, Site-Directed Protein Binding Sigma Factor Species Specificity Spores, Bacterial/growth & development Structure-Activity Relationship Transcription Factors
Chemicals
Bacterial Proteins Sigma Factor Transcription Factors sporulation-specific sigma factors DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shuler M F
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322, USA.
Tatti K M
Wade K H
Moran C P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1995-07-00
Pages
3687-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177084
Subset
IM
Grants
NIGMS NIH HHS · GM39919 · United States
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