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PMID: 7597049 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a nonneuronal isoform of synaptotagmin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 13 ·1995-06-20 ·Pages 5895-9

Hudson AW, Birnbaum MJ

Abstract

Synaptotagmins, which have been found exclusively in neuroendocrine or exocrine tissues, have been implicated in the regulation of secretory vesicle fusion with the plasma membrane. The present paper describes a synaptotagmin isoform (synaptotagmin-5) which exhibits 49% amino acid identity to synaptotagmin-1 and -2. Synaptotagmin-5 mRNA is expressed in rat kidney, adipose tissue, lung, and heart, as well as at higher levels in brain and PC12 cells. Antiserum specific for the synaptotagmin-5 isoform recognizes a protein of about 50 kDa which is about 6-fold more abundant in brain synaptic vesicles than in whole brain membranes.

MeSH Terms
Adipose Tissue/metabolism Amino Acid Sequence Animals Base Sequence Brain/metabolism Calcium-Binding Proteins Cell Line Cloning, Molecular Cricetinae Gene Expression Kidney/metabolism Lung/metabolism Membrane Glycoproteins/biosynthesis,chemistry,isolation & purification Molecular Sequence Data Molecular Weight Muscle, Skeletal/metabolism Myocardium/metabolism Nerve Tissue Proteins/biosynthesis,chemistry,isolation & purification Organ Specificity PC12 Cells Phylogeny RNA, Messenger/analysis,biosynthesis Rats Recombinant Proteins/biosynthesis,chemistry Sequence Homology, Amino Acid Synaptic Vesicles/metabolism Synaptotagmin I Synaptotagmins Transfection
Chemicals
Calcium-Binding Proteins Membrane Glycoproteins Nerve Tissue Proteins RNA, Messenger Recombinant Proteins Synaptotagmin I Syt1 protein, rat Syt5 protein, rat Synaptotagmins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hudson A W
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Birnbaum M J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.13.5895
Published
1995-06-20
Pages
5895-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41608
Subset
IM
Grants
NIDDK NIH HHS · DK39519 · United States
Databases
GENBANK
U26402
External Links
PubMed source DOI: 10.1073/pnas.92.13.5895 PMC Full Text View journal details
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