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PMID: 7596812 Published · ppublish English Journal Article

Compact structures of d(CNG)n oligonucleotides in solution and their possible relevance to fragile X and related human genetic diseases.

Nucleic acids research ·Vol. 23 ·No. 11 ·1995-06-11 ·Pages 1876-81

Mitchell JE, Newbury SF, McClellan JA

Abstract

We show that oligonucleotides of CNG tracts readily adopt compact DNA structures that move unusually fast on gels. Base composition does not explain this, and non-CNG triplets (including GNC) do not form such structures. Chemical probing and melting experiments suggest that the structures probably are not hairpins. Although both long and short tracts can adopt compact structures, the structure formed by longer tracts is more compact than that formed by shorter ones. We note the possibility that such structures may form in vivo, and be instrumental in normal and/or abnormal function of human genes.

MeSH Terms
Base Sequence DNA/chemistry,genetics Fragile X Syndrome/genetics Humans Molecular Sequence Data Molecular Structure Oligonucleotides/chemistry Solutions Temperature
Chemicals
Oligonucleotides Solutions DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mitchell J E
Biophysics Laboratories, University of Portsmouth, UK.
Newbury S F
McClellan J A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/23.11.1876
Published
1995-06-11
Pages
1876-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306957
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/23.11.1876 PMC Full Text View journal details
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