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PMID: 7592394 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Rhizobium NodI and NodJ proteins play a role in the efficiency of secretion of lipochitin oligosaccharides.

Journal of bacteriology ·Vol. 177 ·No. 21 ·1995-11-00 ·Pages 6276-81

Spaink HP, Wijfjes AH, Lugtenberg BJ

Abstract

Thin-layer chromatographic analysis of extracts of D-[1-14C]glucosamine-labelled rhizobia was used to analyze the effects of nodI, nodJ, and nodT on secretion of lipochitin oligosaccharide (LCO) signal molecules. Secretion was analyzed by comparing quantities of radiolabelled LCOs present in the cellular and spent growth medium fractions. A second rapid and sensitive method was introduced to estimate the secreted LCO fractions by using D-[1-14C]glucosamine-labelled cells grown in medium supplemented with chitinase. At various times after induction of LCO synthesis, the quantity of degradation products of LCOs was compared with the amount of nondegraded LCOs. In wild-type strains of Rhizobium leguminosarum biovars viciae and trifolii the nodI and nodJ genes (but not the nodT gene) strongly enhance the secretion of LCOs during the first 5 h after the induction of LCO synthesis. In LCO-overproducing strains the enhancement of secretion was observed only during the first 3 h after induction. At times later than 5 h after induction, a significant influence of the presence of the nodI and nodJ genes on LCO secretion was detectable neither in the wild type nor in LCO-overproducing strains. By using plasmids in which the nodI and nodJ genes are cloned separately under control of a flavonoid-inducible promoter, it was shown that both genes are needed for a wild-type level of LCO secretion. Therefore, these results demonstrate that nodI and nodJ play a role in determining the efficiency of LCO secretion.

MeSH Terms
ATP-Binding Cassette Transporters Bacterial Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Chitin/analogs & derivatives Chromatography, Thin Layer Gene Expression Regulation, Bacterial Genes, Bacterial Lipopolysaccharides/metabolism Membrane Transport Proteins Oligosaccharides/metabolism Rhizobium leguminosarum/genetics,metabolism Species Specificity
Chemicals
ATP-Binding Cassette Transporters Bacterial Proteins Carrier Proteins Lipopolysaccharides Membrane Transport Proteins Oligosaccharides nodI protein, Bacteria nodJ protein, Rhizobium etli Chitin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Spaink H P
Clusius Laboratory, Institute of Plant Molecular Biology, Leiden University, The Netherlands.
Wijfjes A H
Lugtenberg B J
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.21.6276-6281.1995
Published
1995-11-00
Pages
6276-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177469
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.177.21.6276-6281.1995 PMC Full Text View journal details
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