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PMID: 7592347 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of Mu prophage lacking the central strong gyrase binding site: localization of the block in replication.

Journal of bacteriology ·Vol. 177 ·No. 20 ·1995-10-00 ·Pages 5937-42

Pato ML, Karlok M, Wall C, Higgins NP

Abstract

Bacteriophage Mu contains an unusually strong DNA gyrase binding site (SGS), located near the center of its genome, that is required for efficient Mu DNA replication (M. L. Pato, Proc. Natl. Acad. Sci. USA 91:7056-7060, 1994; M. L. Pato, M. M. Howe, and N. P. Higgins, Proc. Natl. Acad. Sci. USA 87:8716-8720, 1990). Replication of wild-type Mu initiates about 10 min after induction of a lysogen, while replication in the absence of the SGS is delayed about an hour. To determine which step in the replication pathway is blocked in the absence of the SGS, we inactivated the SGS by deletion and by insertion and studied the effects of these alterations on various stages of Mu DNA replication. Following induction in the absence of a functional SGS, early transcription and synthesis of the Mu-encoded replication proteins occurred normally. However, neither strand transfer nor cleavage at the Mu genome termini could be detected 40 min after induction. The data are most consistent with a requirement for the SGS in the efficient synapsis of the Mu prophage termini to form a separate chromosomal domain.

MeSH Terms
Bacteriophage mu/enzymology,genetics,growth & development,metabolism Base Sequence Binding Sites/genetics Consensus Sequence DNA Topoisomerases, Type II/metabolism Lysogeny Molecular Sequence Data Mutagenesis Protein Binding Protein Biosynthesis Proviruses/enzymology,genetics,growth & development,metabolism Recombination, Genetic Sequence Deletion Transcription, Genetic Virus Replication
Chemicals
DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pato M L
Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA.
Karlok M
Wall C
Higgins N P
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.20.5937-5942.1995
Published
1995-10-00
Pages
5937-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177422
Subset
IM
Grants
NIGMS NIH HHS · GM33143 · United States
External Links
PubMed source DOI: 10.1128/jb.177.20.5937-5942.1995 PMC Full Text View journal details
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