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PMID: 7591113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Escherichia coli-induced activation of neutrophil NADPH-oxidase: lipopolysaccharide and formylated peptides act synergistically to induce release of reactive oxygen metabolites.

Infection and immunity ·Vol. 63 ·No. 12 ·1995-12-00 ·Pages 4606-12

Karlsson A, Markfjäll M, Strömberg N, Dahlgren C

Abstract

The prevailing view of neutrophil NADPH-oxidase activation during interaction with bacteria is that the production of toxic oxygen metabolites should be directed into the phagosome containing the engulfed prey. However, in this report we show that a common Escherichia coli strain, HB101, may induce a release of neutrophil oxygen metabolites to the extracellular milieu. This phenomenon is dependent on three factors: (i) the mobilization (upregulation) of neutrophil secretory vesicles prior to interaction with the bacteria, (ii) soluble bacterial factors binding to the formylmethionyl-leucyl-phenylalanine receptor and tentatively identified as formylated peptides, and (iii) a bacterium-associated priming factor identified as lipopolysaccharide.

MeSH Terms
Adult Cytoplasmic Granules/metabolism Drug Synergism Enzyme Activation Escherichia coli/immunology Humans Lipopolysaccharides/pharmacology Macrophage-1 Antigen/physiology N-Formylmethionine Leucyl-Phenylalanine/pharmacology NADH, NADPH Oxidoreductases/metabolism NADPH Oxidases Neutrophils/enzymology Polymyxin B/pharmacology Reactive Oxygen Species/metabolism Receptors, Formyl Peptide Receptors, Immunologic/physiology Receptors, Peptide/physiology
Chemicals
Lipopolysaccharides Macrophage-1 Antigen Reactive Oxygen Species Receptors, Formyl Peptide Receptors, Immunologic Receptors, Peptide N-Formylmethionine Leucyl-Phenylalanine NADH, NADPH Oxidoreductases NADPH Oxidases Polymyxin B
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Karlsson A
Department of Medical Microbiology and Immunology, Faculty of Odontology, University of Göteborg, Sweden.
Markfjäll M
Strömberg N
Dahlgren C
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.12.4606-4612.1995
Published
1995-12-00
Pages
4606-12
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173662
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.63.12.4606-4612.1995 PMC Full Text View journal details
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