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PMID: 7591106 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Helicobacter pylori expresses a complex surface carbohydrate, Lewis X.

Infection and immunity ·Vol. 63 ·No. 12 ·1995-12-00 ·Pages 4564-8

Sherburne R, Taylor DE

Abstract

Monoclonal antibodies (MAbs) specific for Lewis X (Lex) reacted with whole cells of Helicobacter pylori NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206 in immunoelectron microscopy and enzyme-linked immunosorbent assay (ELISA) experiments. These MAbs have documented specificity to Lex, whereas MAbs for Lea and Leb were negative in both immunoelectron microscopy and ELISA. H. pylori coccoid forms also reacted with the MAbs, whereas the flagellum lacking the sheath showed no reactivity. The Lex structures were associated with membrane fractions in the ELISA experiments, and silver-stained sodium dodecyl sulfate-polyacrylamide gels confirmed the presence of lipopolysaccharides which reacted with the MAbs in immunoblots. Serum from an H. pylori-infected individual contained immunoglobulins which blocked the binding of the Lex MAbs, indicating that part of the host immune response to H. pylori is to the Lex structure. The ability of this gastric pathogen to mimic an oncofetal antigen (self) could explain the down regulation of anti-H. pylori T-cell response seen in H. pylori-infected individuals.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Carbohydrate Sequence Enzyme-Linked Immunosorbent Assay Helicobacter pylori/immunology,ultrastructure Lewis X Antigen/biosynthesis Mice Microscopy, Immunoelectron Molecular Sequence Data
Chemicals
Antibodies, Monoclonal Lewis X Antigen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sherburne R
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
Taylor D E
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.63.12.4564-4568.1995
Published
1995-12-00
Pages
4564-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173655
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.63.12.4564-4568.1995 PMC Full Text View journal details
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