Home LiteratureArticle Details
PMID: 7581362 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Comparison of the positional cloning methods used to isolate the BRCA1 gene.

Human molecular genetics ·Vol. 4 ·No. 8 ·1995-08-00 ·Pages 1259-66

Harshman K, Bell R, Rosenthal J, Katcher H, Miki Y, Swenson J, Gholami Z, Frye C, Ding W, Dayananth P

Abstract

A critical step in positional cloning is the identification of candidate genes from a large, genetically defined region. Candidate gene isolation by hybrid selection, genomic sequencing, and direct cDNA library screening identified 45 candidate gene fragments (CGFs) from a 600 kb genomic region that contains the BRCA1 gene. These CGFs define a minimum of 15 genes, six of which are newly localized to the BRCA1 region. We present an analysis of the efficiency and the sequences generated for each of these methods. We also compare our CGF set to those reported for the BRCA1 region by three other groups, revealing a surprising lack of overlap among the sets.

MeSH Terms
Breast Neoplasms/genetics Chromosome Mapping Chromosomes, Human, Pair 17/genetics Cloning, Molecular/methods DNA, Complementary/genetics Female Genome, Human Humans Molecular Sequence Data Oncogenes
Chemicals
DNA, Complementary
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Harshman K
Myriad Genetics, Inc., Salt Lake City, UT 84108, USA.
Bell R
Rosenthal J
Katcher H
Miki Y
Swenson J
Gholami Z
Frye C
Ding W
Dayananth P
Article Info
Journal
Human molecular genetics
Abbr.
Hum Mol Genet
ISSN
0964-6906
DOI
10.1093/hmg/4.8.1259
Published
1995-08-00
Pages
1259-66
Language
English
Region
England
NLM ID
9208958
Subset
IM
Grants
NCI NIH HHS · CA 55914 · United States
Databases
GENBANK
U25750, U25751, U25752, U25753, U25754, U25755, U25756, U25757, U25758, U25759, U25760, U25761, U25762, U25763, U25764, U25765, U25766, U25767, U25768, U25769, U25770, U25771, U25772, U25773, U25774, U25775, U25776, U25777, U25778, U25779
External Links
PubMed source DOI: 10.1093/hmg/4.8.1259 View journal details
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