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PMID: 7580932 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A simple "universal" DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification.

PCR methods and applications ·Vol. 4 ·No. 6 ·1995-06-00 ·Pages 368-70

Goldenberger D, Perschil I, Ritzler M, Altwegg M

Abstract

暂无摘要

MeSH Terms

Corynebacterium diphtheriae/classification,genetics,isolation & purification DNA/isolation & purification DNA, Bacterial/isolation & purification Endopeptidase K Enterococcus faecalis/classification,genetics,isolation & purification Escherichia coli/classification,genetics,isolation & purification Humans Indicators and Reagents Polymerase Chain Reaction/methods Polysorbates Sensitivity and Specificity Serine Endopeptidases Sodium Dodecyl Sulfate Staphylococcus aureus/classification,genetics,isolation & purification

Chemicals

DNA, Bacterial Indicators and Reagents Polysorbates Sodium Dodecyl Sulfate DNA Serine Endopeptidases Endopeptidase K

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Goldenberger D

Department of Medical Microbiology, University of Zürich, Switzerland.

Perschil I

Ritzler M

Altwegg M

Article Info

Journal

PCR methods and applications

Abbr.

PCR Methods Appl

ISSN

1054-9803

DOI

10.1101/gr.4.6.368

Published

1995-06-00

Pages

368-70

Language

English

Region

United States

NLM ID

9201445

Subset

External Links

PubMed source DOI: 10.1101/gr.4.6.368

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A simple "universal" DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification.

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